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Research Associate II, Technical Operations

A-Alpha Bio•Seattle, WA
•Onsite

About The Position

A-Alpha Bio is seeking a Research Associate II to support the Technical Operations team in executing its AlphaSeq assay. The role involves DNA and yeast library construction, matings, next-generation sequencing, cell sorting, and flow cytometry to support the interrogation of protein-protein interactions. The ideal candidate is organized, attentive to detail, and interested in executing screening assays at scale. The position is onsite in Seattle, Washington, and is not open to remote applicants. Relocation to Seattle is required. The role is based at A-Alpha Bio’s custom-designed lab and office space in downtown Seattle’s Belltown neighborhood. The company describes its work environment as fast-paced, highly dynamic, and interdisciplinary.

Requirements

  • Bachelor’s degree in a relevant field with at least 2 years of relevant post-baccalaureate laboratory experience in the biotechnology industry, or an equivalent combination of education and experience.
  • Proficiency in standard molecular biology techniques, including DNA extractions, quantitative PCR, Gibson assembly, and fragment analysis.
  • Experience with multi-parametric flow cytometry.
  • Experience in a high-throughput screening or manufacturing environment.
  • Experience working within a LIMS/ELN.
  • Strong work ethic, good organizational skills, team orientation, and excellent attention to detail.
  • Flexibility in day-to-day schedule and willingness to work outside standard hours when necessary, including weekends.

Nice To Haves

  • Operator experience in a regulated environment, such as CLIA or cGxP.
  • Hands-on experience with Illumina or ONT next-generation sequencing platforms.
  • Experience with cell sorting.
  • Experience using yeast as a model organism.
  • Experience using Benchling.
  • Experience using laboratory automation.

Responsibilities

  • Execute DNA library construction.
  • Perform yeast transformation and matings, cell sorting, and flow cytometry.
  • Execute DNA library preparation and sequencing workflows for Oxford Nanopore Technologies and Illumina platforms.
  • Support development and optimization of standard operating procedures for existing and new experimental workflows.
  • Develop foundational understanding and executional expertise in Technical Operations methods.
  • Contribute to project teams, execute project-related tasks, and/or train new Research Associates as needed.

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