Scientist Jobs

About The Position

Requirements

• Candidates with experience in antibody discovery, protein engineering OR expression and validation of biologics are encouraged to apply.
• Previous experience in integrating ML-based approaches with display methods would be preferred.
• Previous experience in library generation would be vital.
• Bachelor's degree in relevant scientific area is required.
• Eight (8) years of relevant work experience is required
• Six (6) years of relevant work experience is required with a Master's degree
• Three (3) years of relevant work experience is required with a PhD

Nice To Haves

• Experience with merging cryo-EM techniques with epitope analysis of antibody repertoires
• Experience with high-throughput automated protein purification systems (e.g. Kingfisher)
• Experience with antibody-related bioinformatic/software packages.
• Expertise in high-throughput molecular biology approaches for construct and library generation.
• Experience with mAb characterization techniques (FACS, BLI, epitope binning).

Responsibilities

• Antibody discovery: Focus on display methods (phage, yeast, mammalian) and experience in building high-diversity libraries from synthetic and animal repertoires.
• Experienced in designing discovery workflows and delivering panel of lead candidates for programs.
• Apply in-vitro display methods for sequence discovery and ability to handle multiple screening arms for antigens.
• Apply NGS methods to mine antibody pools for sequence discovery
• Develop and optimize high-throughput mammalian cell-based production platforms for recombinant antibodies, antigens, and other biologics
• Work closely with mAb discovery platforms to support molecular biology workflows; design and execute construct generation for antibody expression.
• Perform DNA preparations to generate high-quality plasmid material for mammalian cell transfection.
• Establish scalable transient and stable expression workflows in CHO, HEK293, and other mammalian expression systems.
• Purification using AKTA chromatography systems.
• High-Performance Liquid Chromatography Size Exclusion Chromatography (HPLC-SEC) analysis of purified proteins.
• Molecular assessment of early-stage development candidates (polyreactivity ELISA, CE-SDS, SEC-MALS, DSF)
• Maintain accurate and timely ELN data

Benefits

• St. Jude is an Equal Opportunity Employer