Research Services Professional (Open Rank: Entry – Intermediate)

University of Colorado•Aurora, CO

2d•Onsite

About The Position

The Dabertrand Laboratory in the Department of Anesthesiology investigates the control of cerebral blood flow by ion channels and calcium signals in the pericytes, endothelial cells, and smooth muscle cells that constitute the intracerebral microcirculation. We focus on the interaction between neural activity and the microvasculature in different brain regions, in physiological and small vessels disease conditions. We are seeking a Research Services Professional who will perform lab management and conduct/assist with experiments. Experiments include, but not limited to qRT-PCR, western blotting, mammalian cell culture and cell line maintenance, DNA/RNA extraction, electrophoresis, ELISA, proliferation and migration assays, Ca2+ imaging, isolated and pressurized arterioles from mouse brain, patch-clamp electrophysiology on isolated cells, in vivo two-photon microscopy. Responsibilities include solutions and samples preparation, operation and general maintenance of instruments, as well as data collection and analysis. It is an opportunity to learn cutting-edge technologies and to work with investigators on high orbit projects. The candidate should have experience in neuroscience, cardiovascular science or related fields; experience with mouse handling is required, and having microsurgery level/fine motor skills is a plus. The successful candidate should have experience in at least one of these area: molecular biology (RNA extraction, qPCR…), electrophysiology (Patch-clap, EPSP recording), and fluorescence imaging (confocal, two-photon…). This position will work in a collaborative team environment.

Requirements

Bachelor’s degree in biology, molecular biology, chemistry, genetics or related field
A combination of education and related technical/paraprofessional experience may be substituted for the bachelor’s degree on a year for year basis
Applicants must meet minimum qualifications at the time of hire.
One (1) year professional level research experience (Intermediate Level)
Experience with mouse handling is required
Experience in at least one of these area: molecular biology (RNA extraction, qPCR…), electrophysiology (Patch-clap, EPSP recording), and fluorescence imaging (confocal, two-photon…)
Knowledge of laboratory techniques including safety procedures and techniques
Ability to communicate effectively, both in writing and orally
Ability to establish and maintain effective working relationships with employees at all levels throughout the institution
Outstanding customer service skills
Knowledge of basic human anatomy, physiology medical terminology
Ability to interpret and master complex research protocol information

Nice To Haves

Microsurgery level/fine motor skills
Experience with mouse colony maintenance
Specific work experience in molecular, biochemical or cell biology techniques.
Computer literate with proficiency in Microsoft Office Software programs.
Experience with Data Science

Responsibilities

Collaborate with and support Principal Investigators (PI) and other stakeholders with laboratory and research functions
Utilize a variety of laboratory techniques required for research projects
Perform experiments and tabulate and analyze research
Support analytical testing process using standard lab support knowledge in the collection and review of conditions, including preserving samples, completing sample preparation, documenting and communicating results
Perform pre-analytical, analytical and post analytical test process and evaluate results produced
Prepare lab for daily operations, including stocking materials, equipment, safety, cleanliness, and related areas
Provide animal care related to research studies, as necessary
Pick up, transport, and maintain results, samples, documents, materials and other items as needed
Dispose of laboratory materials and samples using well established guidelines and instructions
Review, complete and process logs, documents, databases, and related materials and information
Provide support researching issues and developing recommendations to resolve process, unit and related issues
Assist in the review and implement lab support policies, practices and programs to meet organizational, operations and management needs
Assist with ensuring compliance with applicable laws, rules and regulations; receive guidance and direction or escalate when required
Independently and creatively identify laboratory solutions (Intermediate Level)
Independently Review, develop and implement new or modified laboratory techniques required for research projects (Intermediate Level)
Assist Team Leads, Supervisors and/or management with creation and implementation of processes and procedures and quality improvement initiatives (Intermediate Level)
Assist and train junior team members (Intermediate Level)
IACUC amendment writing
Patience and fine motor skills