Protein Purification Scientist
About The Position
TekWissen is recruiting for a client specializing in biotechnology product development services and serving the scientific community. The client supplies laboratory equipment, chemicals, supplies, and services for healthcare, scientific research, safety, and education. The Scientist II – Protein Purification joins a team performing protein purification in a controlled manufacturing laboratory environment. The role applies knowledge of protein chemistry and downstream processing to execute manufacturing processes, evaluate process performance, troubleshoot technical issues, maintain accurate documentation, and support continuous improvement while meeting product-quality, efficiency, quality-system, and safety requirements. This is a 12-month temporary assignment based onsite in Carlsbad, California, on a first shift schedule, Monday through Friday.
Requirements
- Bachelor's degree in Biology, Chemistry, Biochemistry, or a related scientific discipline, or an equivalent combination of education and relevant experience.
- At least 3 years of relevant protein purification experience in a manufacturing environment or R&D laboratory.
- Mandatory hands-on experience operating FPLC purification systems, specifically Cytiva ÄKTA or Bio-Rad NGC.
- Experience with chromatography techniques, including affinity, ion-exchange, size-exclusion, and/or hydrophobic interaction chromatography.
- Strong understanding of protein chemistry, protein/resin interactions, and chromatography principles.
- Ability to execute protein purification processes, analyze results, and troubleshoot routine technical issues.
- Strong analytical, scientific troubleshooting, and problem-solving skills.
- Ability to execute scientific procedures with limited supervision, interpret results, and communicate findings effectively.
- Excellent attention to detail, documentation practices, and data integrity.
- Strong organizational, collaboration, and communication skills.
- Ability to manage multiple priorities in a fast-paced manufacturing laboratory environment.
- Intermediate Microsoft Excel proficiency.
- Ability to work safely with chemicals and hazardous materials in a controlled laboratory environment.
- Ability to stand for extended periods and regularly lift up to 20 pounds, with occasional lifting of 45 pounds or more with assistance.
- Willingness to work in a +4°C cold room and accommodate varying work schedules as needed.
Nice To Haves
- Experience with Tangential Flow Filtration (TFF) and other downstream protein processing techniques.
- Experience working within a Quality Management System (QMS); familiarity with ISO 9001 and/or GMP environments preferred.
- Experience with Cytiva ÄKTA systems using UNICORN software and/or Bio-Rad NGC chromatography systems.
Responsibilities
- Perform protein purification activities in a laboratory environment according to SOPs and controlled manufacturing documentation.
- Execute column chromatography, Tangential Flow Filtration (TFF), buffer preparation, SDS-PAGE analysis, and other protein characterization techniques.
- Operate Cytiva ÄKTA FPLC systems using UNICORN software and Bio-Rad NGC chromatography systems.
- Analyze process data and chromatograms and monitor process performance using technical knowledge of protein purification and chromatography.
- Identify, investigate, and troubleshoot routine process and equipment issues, escalating complex problems when necessary.
- Maintain accurate Batch Data Records (BDRs), work orders, equipment logs, manufacturing documentation, and associated records.
- Maintain laboratory equipment, materials, and product inventories.
- Support preparation, review, and revision of technical documents, including SOPs and manufacturing batch records.
- Participate in process-improvement initiatives focused on product quality, yield, process robustness, and operational productivity.
- Support process validation, equipment qualification, investigations, and implementation of new or revised manufacturing procedures.
- Collaborate with cross-functional teams to achieve production, quality, and technical objectives.
- Comply with Quality Management System requirements, Environmental Health & Safety programs, and laboratory safety procedures.
- Maintain familiarity with Safety Data Sheets and safe chemical-handling practices.
- Perform additional related duties as assigned.
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What This Job Offers
Career Level
Mid Level
