Core Research Facility Technician III - Gene Expression

University of Florida•Gainesville, FL
•$45,000 - $46,000

About The Position

The Core Research Facility Technician III performs nucleic acid quality control and extraction, SNP analysis, and qPCR and digital PCR for gene expression-related research. The role delivers data to customers, tracks projects submitted through iLab, and uploads completed data to iLab. It also maintains laboratory supplies and equipment, receives incoming packages, and periodically defrosts and cleans laboratory freezers and a refrigerator. Additional duties may be assigned.

Requirements

  • BA/BS in a related scientific field, or an equivalent combination of education and experience.
  • Core or technology-specific licensure and/or certifications where applicable.
  • Complete yearly certificate and compliance training if required.
  • 1–2 years of relevant experience in a scientific research laboratory using standard molecular biology or recombinant DNA methods.
  • Knowledge of and experience working with and maintaining laboratory equipment and supplies.
  • Detail-oriented, with the ability to maintain accurate laboratory records and handle data efficiently.
  • Ability to communicate clearly and effectively with employees and customers.
  • Previous customer service experience.

Nice To Haves

  • BS in Molecular Biology, Biochemistry, Biotechnology, or a related discipline, with 2 years of experience in a multi-user core facility environment focused on analyzing RNA for gene expression studies.
  • Experience operating a Digital PCR System, qPCR, 10x Genomics Chromium technology, and GeneTitan Multichannel System.
  • Experience preparing, assessing the quality of, and processing nucleic acids.
  • Ability to learn new laboratory procedures rapidly.
  • Ability to plan, organize, and coordinate projects.
  • Ability to accept constructive criticism from a supervisor.
  • Self-motivated, positive outlook.

Responsibilities

  • Perform RNA and DNA quality control using Nanodrop and Qubit instruments for quantitation, and Agilent Bioanalyzer or TapeStation instruments.
  • Perform nucleic acid extraction.
  • Process SNP analysis using the GeneTitan Axiom platform and send data to customers.
  • Perform qPCR and digital PCR and deliver data to customers efficiently and accurately.
  • Track iLab-submitted projects and upload data to iLab.
  • Maintain laboratory supply inventory and receive incoming packages.
  • Periodically defrost and clean -80°C and -20°C freezers and the 4°C refrigerator.
  • Perform additional duties as assigned.
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