Research Scientist, Gene Editing Core

Mass General Brigham • Boston, MA

2d • $80,000 - $110,000 • Onsite

About The Position

The Research Scientist will support and drive genome-editing and CRISPR perturbation projects within the Gene Editing Core (GEC) of Mass General Brigham’s Gene and Cell Therapy Institute. The GEC partners with academic and industry collaborators to support gene therapy development from initial concept through validation, providing consulting, experimental design, assay development, and screening capabilities. The role contributes to research aimed at advancing genetic therapies and works with internal and external collaborators in a state-of-the-art gene-editing core.

Requirements

• Ph.D. in Molecular Biology, Cell Biology, Genomics, Bioengineering, or a related field.
• Strong expertise in molecular biology techniques, including PCR, cloning, qPCR, ddPCR, and NGS library preparation.
• Extensive experience with CRISPR-based genome editing and perturbation-screening technologies.
• Hands-on experience with molecular cloning methods, including Gibson Assembly and Golden Gate cloning, particularly for CRISPR library generation.
• Familiarity with off-target analysis techniques and next-generation sequencing.
• Ability to analyze sequencing data and interpret gene-editing efficiency and specificity.
• Excellent organizational skills and attention to detail, with the ability to manage multiple projects simultaneously.
• Strong written and verbal communication skills.

Nice To Haves

• Experience with prime-editing and base-editing workflows.
• Expertise in CRISPR screening approaches, including CRISPR KO, CRISPRi, and CRISPRa.
• Experience in a core facility or industry setting providing genome-editing services.
• Familiarity with bioinformatics tools for NGS data analysis, such as Python, R, or Bioconductor.
• Hands-on experience with iPSC culture and differentiation.

Responsibilities

• Develop and optimize CRISPR-based genome-editing techniques for various cell types, including iPSCs and primary cells.
• Perform molecular biology assays, including molecular cloning, PCR, ddPCR, RT-qPCR, NGS library preparation, and quality control assessments.
• Conduct NGS-based off-target analyses to assess genome-editing outcomes.
• Lead and execute genetic perturbation experiments, including pooled and arrayed CRISPR screens.
• Collaborate with internal and external partners to design, troubleshoot, and optimize gene-editing experiments.
• Maintain detailed, organized experimental records in electronic lab notebooks.
• Stay current with emerging technologies in genome editing, CRISPR perturbation, and functional genomics.
• Train and mentor junior staff and research technicians in gene-editing workflows and molecular biology techniques.
• Contribute to scientific publications and presentations at internal and external meetings.
• Perform other duties as assigned.

Benefits

• Comprehensive benefits
• Career advancement opportunities
• Recognition programs
• Professional development opportunities, including conference participation and networking

What This Job Offers

• Job Type: Full-time
• Career Level: Senior
• Education Level: Ph.D. or professional degree