Research Technician II - Bosse Lab

Children’s Hospital of Philadelphia•Philadelphia, PA

1d•Onsite

About The Position

This entry-level research technical role participates in a planned research projects by performing research protocols, experimental procedures and other laboratory tasks under direct supervision. May occasionally exercise minimal discretion and judgment in execution and interpretation of experiments, and of data derived. Will conduct translational pediatric cancer and immuno-oncology research under the direction/guidance of Dr. Kris Bosse in collaboration with the St. Baldricks-Stand Up To Cancer Immunogenomics Team. Dr. Bosse’s laboratory is focused on developing immunotherapeutic strategies targeting tumor integral cell surfaces molecules in neuroblastoma and other incurable pediatric cancers. There is also a major focus in the Bosse Laboratory on utilizing circulating tumor DNA (ctDNA) to define mechanisms of therapeutic resistance and disease progression in neuroblastoma. The research technician will join a growing translational research team based in his laboratory, and work in collaboration with several other laboratories at CHOP/Penn. Ongoing projects include translating current immunotherapies in the lab across pediatric cancers, new immunotherapeutic target discovery in neuroblastoma and other pediatric cancers, chimeric antigen receptor T cell therapy and antibody-drug conjugate development, defining mechanisms of tumor cell intrinsic and extrinsic immune escape, deciphering the key biologic functions of prioritized immunotherapeutic targets and using serial neuroblastoma preclinical models to define mechansisms of therapeutic resistance. Diverse molecular biology, genomic, and in vitro and in vivo laboratory techniques will be employed. The ideal candidate will (1) Work independently and be self-directed with guidance from the principal investigator, (2) Develop new experimental procedures and hypotheses for further investigation and will help the principle investigator publish and present research results, (3) Assist with the management of laboratory operations, (4) Collaborate and interact with other researchers within the laboratory, institution and at other institutions, (5) Consistently exercise discretion and judgment in execution, interpretation of experiments, and of data derived, and (6) Have some laboratory experience with techniques such as cell culture, RNA purification, PCR, cloning, Western blotting, in vitro functional assays, and flow cytometry. Experience working with mouse models would also be a plus. At least a 2-year commitment is required. Will have multiple opportunities to present work at national and international meetings and will be involved in writing manuscripts. Laboratory environment includes a diverse range of scientists and trainees at all career levels, including multiple research technicians doing a 2-3 year laboratory experience prior to applying to professional (e.g. Graduate or Medical) school.

Requirements

High School Diploma / GED Required
At least one (1) year of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience Required
Intermediate understanding of lab procedures, protocols, and equipment.
Basic exposure to lab specific experiments / techniques and scientific analysis
Excellent verbal and written communications skills
Excellent organizational skills
Solid critical thinking / problem-solving skills
Solid analytical skills

Nice To Haves

Associate's Degree Preferred
Bachelor's Degree Preferred
Bachelor’s Degree in biological or chemical sciences or another scientific discipline Ppreferred
At least two (2) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience Preferred
Experience working with mouse models would also be a plus.

Responsibilities

Performs routine tests / experiments under the direction of principal investigator, supervisor or other designee
Performs research procedures on experimental samples as directed
Records and checks experiments/ results for completeness and accuracy
Performs calculations and maintains appropriate log and lab notebooks
Performs tasks that require familiarity with fundamental techniques and lab equipment
May assist with planning specific research protocols and procedures
Maintains office, lab area, solutions, reagents, supplies and equipment, disposes of specimens, media and other materials (using appropriate procedures)
Assists in care and maintenance of lab equipment
May coordinate schedules of tests and procedures
Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques
Records results, organizes data and maintains lab notebook in an organized manner
Compiles and helps analyze data, prepares charts, graphs, and results of studies as directed
Conducts literary searches as directed