Research Technician II - Bosse Lab

Children's Hospital of Philadelphia•Philadelphia, PA

1d•Onsite

About The Position

This entry-level research technical role participates in planned research projects by performing research protocols, experimental procedures, and other laboratory tasks under direct supervision. The role involves conducting translational pediatric cancer and immuno-oncology research under the direction of Dr. Kris Bosse, in collaboration with the St. Baldricks-Stand Up To Cancer Immunogenomics Team. The Bosse Laboratory focuses on developing immunotherapeutic strategies targeting tumor integral cell surface molecules in neuroblastoma and other pediatric cancers, as well as utilizing circulating tumor DNA (ctDNA) to define mechanisms of therapeutic resistance and disease progression. The research technician will join a growing translational research team and collaborate with other laboratories at CHOP/Penn. Ongoing projects include translating current immunotherapies, discovering new immunotherapeutic targets, developing CAR T-cell therapy and antibody-drug conjugates, defining mechanisms of immune escape, deciphering key biologic functions of targets, and using preclinical models to define mechanisms of therapeutic resistance. Diverse molecular biology, genomic, and in vitro and in vivo laboratory techniques will be employed. The ideal candidate will work independently with guidance, develop new experimental procedures, assist with laboratory management, collaborate with other researchers, exercise discretion and judgment in experiments and data interpretation, and have some laboratory experience with techniques such as cell culture, RNA purification, PCR, cloning, Western blotting, in vitro functional assays, and flow cytometry. Experience with mouse models is a plus. A minimum 2-year commitment is required, with opportunities to present work at national and international meetings and be involved in manuscript writing. The laboratory environment includes a diverse range of scientists and trainees at all career levels.

Requirements

At least one (1) year of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience Required
Intermediate understanding of lab procedures, protocols, and equipment.
Basic exposure to lab specific experiments / techniques and scientific analysis
Excellent verbal and written communications skills
Excellent organizational skills
Solid critical thinking / problem-solving skills
Solid analytical skills

Nice To Haves

Associate's Degree Preferred
Bachelor's Degree Preferred
Bachelor’s Degree in biological or chemical sciences or another scientific discipline Ppreferred
At least two (2) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience Preferred
Experience working with mouse models would also be a plus.
Work independently and be self-directed with guidance from the principal investigator
Develop new experimental procedures and hypotheses for further investigation and will help the principle investigator publish and present research results
Assist with the management of laboratory operations
Collaborate and interact with other researchers within the laboratory, institution and at other institutions
Consistently exercise discretion and judgment in execution, interpretation of experiments, and of data derived
Have some laboratory experience with techniques such as cell culture, RNA purification, PCR, cloning, Western blotting, in vitro functional assays, and flow cytometry.

Responsibilities

Performs routine tests / experiments under the direction of principal investigator, supervisor or other designee
Performs research procedures on experimental samples as directed
Records and checks experiments/ results for completeness and accuracy
Performs calculations and maintains appropriate log and lab notebooks
Performs tasks that require familiarity with fundamental techniques and lab equipment
May assist with planning specific research protocols and procedures
Maintains office, lab area, solutions, reagents, supplies and equipment, disposes of specimens, media and other materials (using appropriate procedures)
Assists in care and maintenance of lab equipment
May coordinate schedules of tests and procedures
Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques
Records results, organizes data and maintains lab notebook in an organized manner
Compiles and helps analyze data, prepares charts, graphs, and results of studies as directed
Conducts literary searches as directed