Protein Purification & Expression Scientist

Eurofins USA PSS Insourcing Solutions•West Point, PA

About The Position

The protein expression and purification team provides protein vaccine candidates and critical assay reagents to support target validation and identification during early discovery. This position primarily supports small-scale screening of protein antigens using 30 mL cultures and large-scale purification of recombinant proteins and nanoparticles using 5 L cultures. Weekly work includes preparing buffers, maintaining instruments, tracking work orders, sanitizing chromatographic columns, keeping detailed records, preparing reports, and maintaining inventory. The role may also provide hands-on support to the protein expression group and involves collaboration with the protein characterization group and other teams.

Requirements

A BS with 3–5 or more years of post-graduate work experience, or an MS with 1–2 years of graduate and post-graduate laboratory experience, in protein expression and purification of recombinant proteins.
Demonstrated expertise in research-scale (up to 100 mg) affinity-based FPLC protein purification chromatography.
Knowledge of affinity, charge-based, and size-exclusion protein separation techniques.
Proficiency in protein characterization techniques and protein-based assays, including Bradford protein quantitation, UV-Vis spectroscopy, SDS-PAGE, and Western blotting.
Strong command of aseptic technique and sterile cell culture in a biosafety cabinet.
Effective communication skills and the ability to work independently and collaboratively.
Meticulous attention to detail when following SOPs.
Comprehensive understanding of Microsoft Office applications.
Authorization to work indefinitely in the United States without restrictions.

Nice To Haves

Knowledge of protein characterization by HPLC or UPLC.
Proficiency in automated, tip-based chromatography systems.
Experience in recombinant protein expression.
Experience with orthogonal protein characterization techniques, such as spectroscopy or MS.
Experience with bacterial or mammalian cell culture, including cell passaging, cell counting, determination of cell density, and preparation of dilutions.

Responsibilities

Support small-scale screening of protein antigens using 30 mL cultures.
Purify recombinant proteins and nanoparticles at large scale using 5 L cultures.
Prepare buffers and maintain instruments.
Track work orders and sanitize chromatographic columns.
Keep detailed records and prepare reports.
Maintain inventory.
Provide occasional hands-on support to the protein expression group.
Collaborate with the protein characterization group and other teams.