J1 Research Collaborator - Retzky College of Pharmacy - Pharmaceutical Sciences - Polikanov Lab

University of Illinois Chicago•Chicago, IL

4d•Onsite

About The Position

The J1 Research Collaborator will conduct research aimed at elucidating the structural and molecular basis of bacterial ribosome function and its inhibition by small-molecule compounds. The research will involve a combination of structural, biochemical, and molecular biology approaches, including X-ray crystallography and cryo-electron microscopy (cryo-EM), to investigate mechanisms of protein synthesis and the action of ribosome-targeting antibiotics.

Requirements

Bachelor’s degree in chemistry, biochemistry, biophysics, or a related field.
Minimum 3 years of laboratory research experience.

Nice To Haves

Master’s degree or higher is preferred.
Practical skills with the most standard methods currently used in molecular biology, and biochemistry (such as protein expression, purification).
Previous experience with lab work.
Previous experience in structural biology (cryo-EM or X-ray crystallography) is desired.
Previous experience in RNA biology is desired.

Responsibilities

Conduct and analyze experiments related to bacterial ribosome structure, function, and inhibition by small-molecule compounds.
Purify bacterial ribosomes and prepare additional components required for biochemical and structural studies, including recombinant proteins and tRNAs.
Prepare ribosomal complexes and other biological samples for structural studies using X-ray crystallography and cryo-EM.
Prepare culture media, buffers, solutions, and reagents according to established protocols.
Perform standard molecular biology and biochemical procedures, including recombinant protein expression and purification.
Analyze, interpret, document, and organize experimental data and research results.
Maintain accurate and detailed records of experiments, procedures, protocols, and results.
Assist with training graduate students and other laboratory personnel in research methods, proper use of laboratory equipment, and record keeping.
Maintain laboratory equipment and ensure that equipment and glassware are properly used, cleaned, and maintained.
Maintain an organized laboratory environment and follow appropriate laboratory safety and contamination-control procedures.
Monitor and maintain inventories of chemicals, reagents, consumables, and other laboratory supplies.
Perform other related duties and participate in special projects as assigned.