Part-time Staff Research Associate

UCSF • San Francisco, CA

14d • $31 - $33

About The Position

The lab studies the molecular mechanisms of lung development and repair. The Staff Research Associate will primarily conduct phenotypic and molecular analyses of mouse models of lung development and repair. The work involves tissue collection, processing, and analysis; molecular biology and biochemistry techniques; cell culture and cell-based studies; and data analysis, including transcriptomics and epigenomics. Duties are performed under supervision and are assigned for a total of 16 hours.

Requirements

• BA/BS in a related science and knowledge of, or experience with, the basic techniques or methods required by the position; or an equivalent combination of education and experience.
• Attention to detail.
• Ability to follow instructions and protocols.
• Ability to keep track of animal numbers during experimental manipulations.
• Time management skills to complete assigned tasks within 16 hours.
• Excellent written and verbal communication skills.
• Excellent interpersonal skills.
• Excellent organizational skills to support smooth laboratory operations by maintaining adequate supplies and equipment operation.
• Ability to handle unpleasant elements and wear protective clothing or equipment.

Nice To Haves

• Knowledge and experience in molecular biology and biochemistry.
• Knowledge and experience in cell-based studies.
• Knowledge and experience in animal studies.
• Knowledge and experience in data analysis.

Responsibilities

• Perform molecular biology and biochemistry experiments under supervision, including genomic DNA, RNA, and protein isolation; agarose gel electrophoresis; DNA isolation from agarose gels; DNA ligation and transformation; mini- and maxi-preparation of DNA; PCR and qPCR; Western blotting; and sgRNA design.
• Perform phenotypic and molecular analyses of mouse models of lung development and repair, including general mouse husbandry, lung dissection and perfusion, fixation, embedding and sectioning of lungs, antibody staining of lung tissues, and EdU and TUNEL staining.
• Perform cell culture and cell-based studies, including lung cell isolation, organoid culture, transduction of lung cells with candidate genes or libraries, and CRISPR and CRISPR screens of lung cells.
• Perform data analysis involving bulk RNA-seq, scRNA-seq, multi-omics, ATAC-seq, spatial transcriptomics, CUT&RUN, and CRISPR screens.

What This Job Offers

• Job Type: Part-time