Part-time Staff Research Associate

University of California, San Francisco • San Francisco, CA

12d

About The Position

The lab studies the molecular mechanisms of lung development and repair. The part-time Staff Research Associate will primarily conduct phenotypic and molecular analyses of mouse models of lung development and repair. The role involves tissue collection, processing, and analysis; molecular biology and biochemistry techniques; cell culture and cell-based studies; and data analysis, including transcriptomics and epigenomics. Work is performed under supervision.

The position allocates 25% of time to molecular biology and biochemistry experiments, 25% to mouse-model phenotypic and molecular analyses, 25% to cell culture and cell-based studies, and 25% to data analysis.

Requirements

• BA/BS in a related science and knowledge of, or experience with, the basic techniques or methods required by the position; or an equivalent combination of education and experience.
• Attention to detail.
• Ability to follow instructions and protocols.
• Ability to keep track of animal numbers during experimental manipulations.
• Time management skills to complete assigned tasks within 16 hours.
• Excellent written and verbal communication skills.
• Excellent interpersonal skills.
• Excellent organizational skills to support smooth laboratory operations by maintaining adequate supplies and equipment operation.
• Ability to handle unpleasant elements and wear protective clothing or equipment.

Nice To Haves

• Knowledge and experience in molecular biology and biochemistry.
• Knowledge and experience in cell-based studies.
• Knowledge and experience in animal studies.
• Knowledge and experience in data analysis.

Responsibilities

• Under supervision, perform molecular biology and biochemistry experiments, including genomic DNA, RNA, and protein isolation; agarose gel electrophoresis; isolation of DNA from agarose gel; DNA ligation and transformation; mini- and maxi-preparation of DNA; PCR and qPCR; Western blotting; and sgRNA design.
• Under supervision, perform phenotypic and molecular analyses of mouse models of lung development and repair, including general mouse husbandry, lung dissection and perfusion, lung fixation, embedding and sectioning, antibody staining of lung tissues, and EdU and TUNEL staining.
• Under supervision, perform cell culture and cell-based studies, including lung cell isolation, organoid culture, transduction of lung cells with candidate genes or libraries, and CRISPR and CRISPR screens of lung cells.
• Under supervision, perform data analysis involving bulk RNA-seq, scRNA-seq, multi-omics, ATAC-seq, spatial transcriptomics, CUT&RUN, and CRISPR screens.

What This Job Offers

• Job Type: Part-time
• Career Level: Entry Level