Senior Scientist, Biology

Flagship Pioneering, Inc.•Cambridge, MA

About The Position

We are seeking a highly motivated and experienced Senior Scientist to join our research team. The successful candidate will possess deep expertise in cell isolation and sorting, in vivo/ in vitro mouse studies, gene-silencing technologies (siRNA), flow cytometry and advanced microscopy techniques. This individual will play a key role in designing, executing, and interpreting preclinical studies to therapeutic programs. Depending on experience and demonstrated leadership, the candidate may lead research initiatives, mentor team members, manage external collaborations, and contribute to scientific and program strategy.

Requirements

Ph.D. in Cell Biology, Genetics, Molecular Biology, Pharmacology, Immunology, Biomedical Sciences, or a related field with 6+ years of relevant experience for Senior Scientist roles; 8+ years of industry experience and demonstrated scientific leadership for Principal Scientist roles.
Alternatively, an M.S. degree with substantial relevant industry experience.
Strong expertise in flow cytometry, including cell sorting (FACS), panel design, troubleshooting, and data analysis using FlowJo or equivalent software.
Hands-on experience with cell isolation techniques, including tissue dissociation and magnetic bead-based separation.
Extensive experience with mouse models and in vitro experimental techniques.
Demonstrated expertise in siRNA design, delivery, optimization, and functional validation.
Hands-on experience with microscopy platforms, including fluorescence, confocal, live-cell, and high-content imaging systems.
Strong quantitative data analysis, experimental design, and statistical analysis skills.
Excellent communication, presentation, and scientific writing skills.
Ability to work independently and collaboratively in a fast-paced, multidisciplinary environment.

Nice To Haves

Experience in translational research, drug discovery, or preclinical therapeutic development.
Experience in biotechnology or pharmaceutical industry settings.
Experience with cell profiling, phenotyping, and functional assays using primary mouse and human cells, as well as established cell lines.
Experience with single-cell and/or spatial biology technologies, including scRNA-seq, CITE-seq, or spatial transcriptomics.
Experience managing CROs, external collaborators, and cross-functional project teams.
Proficiency with additional molecular and cellular biology techniques, including: qPCR, Western blotting, ELISA and multiplex cytokine assays, RNA-seq and transcriptomic analysis, Gene silencing and gene-editing approaches, Protein overexpression systems, Immunohistochemistry (IHC), immunofluorescence (IF), and advanced flow cytometry applications
Experience with image analysis software such as ImageJ/Fiji, CellProfiler, Imaris, or equivalent platforms.
Experience with computational analysis of flow cytometry, imaging, or transcriptomic datasets.
Track record of scientific publications, patents, and/or successful advancement of therapeutic programs.

Responsibilities

Design, execute, and analyze complex in vitro and ex vivo experiments to support discovery and preclinical research programs.
Lead and perform multi-parameter flow cytometry assays, including panel design, optimization, troubleshooting, and data interpretation.
Perform and optimize cell isolation and cell sorting (e.g., FACS, MACS) from various tissues and biological samples.
Execute in vivo mouse studies, including disease model development, dosing, tissue collection, and phenotypic characterization.
Develop and apply siRNA-based approaches for target validation, pathway interrogation, and mechanistic studies.
Design and perform microscopy-based experiments, including fluorescence, confocal, live-cell, and high-content imaging, and analyze imaging datasets.
Utilize complementary molecular and cellular biology techniques to support target validation and mechanism-of-action studies.
Design, analyze, and interpret cell profiling experiments using flow cytometry, imaging, and molecular approaches.
Contribute to biomarker discovery, translational research efforts, and preclinical candidate characterization.
Analyze, interpret, and present experimental findings to cross-functional teams and senior leadership.
Generate technical reports, presentations, study summaries, and scientific documentation to support program advancement.
Collaborate effectively with internal teams, CROs, academic collaborators, and external partners.
Mentor junior scientists and contribute to a collaborative, innovative research environment.
For Senior Scientist and Principal Scientist levels, provide scientific leadership, contribute to research strategy, and lead cross-functional initiatives.
Maintain accurate experimental records and ensure compliance with institutional, ethical, and regulatory guidelines.