Scientist, Biology-Discovery
About The Position
The successful candidate will join the protein expression and purification team in the department of Infectious Diseases and Vaccines (IDVAX). The protein expression and purification team provides protein vaccine candidates and critical assay reagents for IDVAX programs in support of target validation and identification in the early discovery space. The primary focus for this position will be small-scale (30 ml culture) screening of reagent and vaccine antigens and large-scale (5 L culture) purification of recombinant proteins and nanoparticles. The candidate will work closely with the protein expression group, collaborate with the protein characterization group, as well as IDVAX program teams.
Requirements
- Proficiency in automated, tip-based chromatography systems and research-scale FPLC protein purification chromatography
- Demonstrated knowledge in affinity, charge-based, and size exclusion separation techniques
- Ability to closely follow SOPs
- Knowledge in developing purification strategies for novel proteins
- Proficiency in different protein characterization techniques and protein-based assays such as, Bradford protein quantitation, UV-VIS spectroscopy, SDS-PAGE, Western blotting
- Familiarity with Microsoft office applications
- Effective communication skills
- Ability to work independently and in collaboration with others
- Attention to details
- Interest in related scientific fields and drug discovery
- BS (with 2 or more years of post-graduation work experience) or MS (with 1 year or more years of graduate or post-graduation laboratory experience) in biology, chemistry, or another scientific field
- Work experience can be in either academic or industry setting
Nice To Haves
- Knowledge of endotoxin measurements
- Protein analytical methods such as fluorimetry, infrared spectroscopy, circular dichroism spectroscopy, light scattering, ultracentrifugation, or gradient purification
- Experience in recombinant protein expression in either bacterial, insect or mammalian cell lines
- Basic knowledge in sterile techniques
- Basic knowledge of RNA biochemistry
Responsibilities
- Small-scale (30 ml culture) screening of reagent and vaccine antigens
- Large-scale (5 L culture) purification of recombinant proteins and nanoparticles
- Preparation of buffers
- Maintenance of purification instruments
- Sanitization of chromatographic columns
- Detailed notebook keeping
- Preparations of reports
- Inventory maintenance
Benefits
- Medical
- Dental
- Vision healthcare
- Other insurance benefits (for employee and family)
- Retirement benefits, including 401(k)
- Paid holidays
- Vacation
- Compassionate and sick days
- Annual bonus
- Long-term incentive
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What This Job Offers
Job Type
Full-time
Career Level
Mid Level
Education Level
Associate degree
