Scientist Bioscience - Proteomics

the Client•Cambridge, MA

10d•Onsite

About The Position

We are seeking a highly motivated and talented scientist to join the Chemical Biology and Proteomics group within Discovery Sciences. As a Research Scientist on a 12-month fixed-term contract, you will apply proteomics to advance drug discovery. You will work as an integral member of a cross-functional team to interrogate mechanisms of action for therapeutic agents, and to propose and validate biological hypotheses for agents within the cardiovascular, renal and metabolism (CVRM) area. If you're inspired by the possibilities of science to make a difference and ready to discover what you can do – join us.

Requirements

Hands-on proteomics experience (from graduate, postdoctoral, or industry work)
Mammalian cell culture experience
Data analysis skills
Knowledge and hands-on experience with proteomic sample preparation
Experience employing cell-based and proteomic assays to understand small molecule mechanisms of action
Demonstrated ability to work effectively both independently and collaboratively, contribute to scientific discussions, solve problems, and deliver high-quality results within agreed timelines
Similar workflows including mammalian cell culture, protein conjugation, purification, and UV-Vis spectroscopy characterization
Carrying out advanced mass spectrometry-based proteomics with affinity enrichments
Expected to operate with significantly higher independence than an Associate Scientist level

Nice To Haves

Experience developing proximity ligation assays (PLA) or related proximity-based protein-protein interaction validation assays
Experience using LC-MS/MS platforms and semi-automated instrumentation
Experience using proteomics search and analysis tools, such as Spectronaut, Proteome Discoverer, MaxQuant, Skyline, and Xcalibur

Responsibilities

Design and execute discovery proteomic assays using mass spectrometry to support mechanistic studies, identify targets, and elucidate biological regulators of disease biology.
Perform proximity biotinylation studies in cells, using established automated affinity purification workflows and data-independent acquisition (DIA) mass spectrometry methods.
Perform target validation by developing proximity ligation assays (PLA) and cell-based assays to assess protein-protein interactions using chemical tools and confocal microscopy.
Generate protein conjugates and characterize protein labeling by UV-Vis spectroscopy.
Analyze and interpret experimental data using established bioinformatic pipelines.
Maintain detailed and accurate electronic laboratory notebooks.
Represent the CBP team, communicate with project discipline leads, and contribute to the generation of mechanistic hypotheses through interpretation of proteomics data.