Scientist, Bioconjugation

Manifold Bio•Boston, MA

9d•Onsite

About The Position

Manifold Bio is a platform biotechnology company pioneering AI-guided protein design and massively multiplexed in vivo screening to unlock tissue-targeted medicines and organism-scale models of living systems. Using proprietary molecular barcoding technology, we screen hundreds of thousands of protein designs simultaneously in living systems, producing in vivo-validated datasets at a scale no one else can match. The datasets power our computational models, which leads to better drug designs, creating a flywheel that gets stronger with every campaign. Our team of protein engineers, biologists, and computational scientists works across this full stack to pursue programs both internally and with leading pharma companies. Position Manifold Bio is seeking a scientist experienced in protein chemistry, oligonucleotide chemistry, and bioconjugation. You will be responsible for designing and executing bioconjugation strategies for protein-oligonucleotide conjugates, as well as bioconjugated fluorophores, tags, and peptides. In addition to performing, optimizing, and troubleshooting bioconjugation in the lab, you will oversee contract research organizations executing specialized or large-scale bioconjugation projects. You will consistently execute core bioconjugation protocols, proactively contribute to workflow optimization, and bring your excitement for rigorous science and troubleshooting to our growing cross-functional team. You will join a team with deep experience in protein chemistry, RNA biology, and biology, and work closely with a highly interdisciplinary team of creative experimental and computational scientists to advance our tissue-targeted oligomedicines pipeline.

Requirements

PhD in Biochemistry, Chemistry, Molecular Biology, or a related scientific field
Minimum of 3+ years of hands-on wet-lab experience in protein production, purification, and biochemistry
Proficiency in basic biochemical techniques (e.g., protein quantification, gel electrophoresis, chromatography)
Proven ability to troubleshoot biochemical assays and optimize experimental protocols
Experience with bioconjugation chemistry principles and methodologies (e.g., cysteine-based, lysine-based, and/or enzymatic conjugation strategies)
Familiarity with oligonucleotide chemistry and handling

Nice To Haves

Experience with high-throughput screening or automation
Familiarity with analytical techniques for biomolecule characterization (e.g., mass spectrometry, DLS, BLI, SPR)
Experience establishing and troubleshooting enzymatic assays
Demonstrated leadership or mentorship of junior scientists
Strong presentation skills and design sensibility

Responsibilities

Design, execute, and troubleshoot bioconjugation reactions for protein-oligonucleotide conjugates and other bioconjugated fluorophores, tags, and peptides
Purify and characterize bioconjugated products using standard biochemical techniques
Build and maintain a parallelized, efficient workflow to support in vivo target engagement evaluation of multiple new candidate tissue-targeting shuttles using tool siRNA payloads
Oversee contract research organizations (CROs) performing specialized or large-scale bioconjugation projects
Proactively identify and drive optimization of existing bioconjugation and analytical workflows
Maintain accurate, detailed experimental records and contribute to scientific discussions and experimental design