Research Technologist – iPSC Cell Culture and Genome Engineering

Penn State University•Tallahassee, FL

8d•$40,608 - $58,836•Onsite

About The Position

We are seeking a highly motivated Research Technologist to join a cardiovascular research laboratory focused on human induced pluripotent stem cell (iPSC) models of cardiac diseases inherited. The successful candidate will play a key role in establishing and maintaining iPSC cultures. This position offers an excellent opportunity to gain hands-on experience with cutting-edge stem cell technologies, genome engineering, and translational cardiovascular research in a collaborative academic environment.

Requirements

Experience with mammalian cell culture.
Strong organizational skills and attention to detail.
Excellent written and verbal communication skills.
Ability to work independently while contributing effectively within a collaborative research team.
Bachelor's Degree 1+ years of relevant experience; or an equivalent combination of education and experience accepted

Nice To Haves

Bachelor's or master’s degree in Cell Biology, Molecular Biology, Genetics, Biomedical Sciences, Biotechnology, or a related field.
Experience culturing human iPSCs or embryonic stem cells.
Experience with stem cell differentiation, particularly cardiac differentiation.
Familiarity with PCR, cloning, DNA sequencing, and molecular biology techniques.
Experience with fluorescence microscopy, flow cytometry, or next-generation sequencing is desirable.
Prior experience in cardiovascular, stem cell, or genetic disease research.
Strong motivation to learn new techniques.
Excellent problem-solving and troubleshooting skills.
Ability to manage multiple projects simultaneously.
Commitment to maintaining rigorous scientific standards and reproducible research.
Interest in translational medicine and cardiovascular genetics.

Responsibilities

Perform routine maintenance, expansion, cryopreservation, and quality control of human iPSC lines.
Differentiate iPSCs into cardiomyocytes and other relevant cell types following established protocols.
Isolate and expand single-cell clones and perform molecular validation using PCR, Sanger sequencing, and related techniques.
Conduct molecular and cellular assays including DNA/RNA extraction, qPCR, Western blotting, immunofluorescence staining, and flow cytometry as needed.
Maintain accurate laboratory records, experimental documentation, and electronic databases.
Prepare reagents, maintain cell culture supplies, and ensure compliance with laboratory safety procedures.
Assist in data analysis, figure preparation, and presentation of research findings.
Participate in laboratory meetings and contribute to collaborative research projects.