Research Technician

Drexel•Philadelphia, PA

2d•Onsite

About The Position

The Matt lab explores the inflammatory impact of psychiatric drugs on patient-derived human immune cells and iPSC-derived myeloid and brain models in the context of HIV infection. The majority of our experiments use high-content imaging to perform high-throughput analysis of large, heterogenous cell populations and molecular targets in response to many distinct stimuli. Administratively, this individual is responsible for a variety of logistical and maintenance tasks as needed to keep lab operations running smoothly. These include but are not limited to ordering supplies, maintaining lab inventory and managing lab instruments. Scientifically, the individual hired for this position will be heavily involved in the isolation, culture and differentiation of immune cells from human blood. This individual will also be responsible for characterizing the cells obtained from each isolation using standard molecular biology techniques such as qPCR, Western blotting and flow cytometry. This individual will also be actively assisting Dr. Matt with specific projects involving HIV infection, high content imaging and analysis, qPCR and Western blotting, and will also help assist other lab members with their specific projects as time permits. A successful candidate will join the Matt lab in the Department of Pharmacology and Physiology at Drexel University College of Medicine as a research technician. The ideal candidate will have 1+ years of research experience, be proactive, able to work well with groups, highly organized and willing to learn. This individual must be comfortable being trained to work with HIV in BSL-2+ settings. Individual should be experienced with academic software such as Microsoft Office (Word / Excel / Powerpoint), Lab Archives, Biorender, Prism, Adobe Illustrator, and similar programs as well as with cell culture and standard molecular biology techniques (Western blotting, qPCR, immunofluorescent staining, flow cytometry). This position is grant-funded; employment is contingent upon the continued availability of those funds.

Requirements

Minimum of a Bachelor's Degree in Biology/Neuroscience/Immunology or the equivalent combination of education and work experience.
Minimum of 1+ years of experience.
Experienced with academic software such as Microsoft Office (Word / Excel / Powerpoint), Lab Archives, Biorender, Prism, Adobe Illustrator, and similar programs.
Experienced with cell culture and standard molecular biology techniques (Western blotting, qPCR, immunofluorescent staining, flow cytometry).
Comfortable being trained to work with HIV in BSL-2+ settings.

Nice To Haves

Be proactive.
Able to work well with groups.
Highly organized.
Willing to learn.

Responsibilities

Experiment prep, molecular assays.
Work directly with Dr. Matt setting up and executing specific experiments.
Prepare reagents and stocks.
Regular isolation and culture of macrophages from fresh and frozen human blood samples.
Differentiation and culture of iPSC-derived myeloid cells.
Perform molecular assays (flow cytometry, qPCR, immunofluorescent staining, Western Blots) to assist Dr. Matt and other lab members with their projects as time permits.
Monitor and maintain inventories of laboratory reagents, supplies and samples.
Order new reagents, supplies, and equipment as necessary.
Maintaining lab space in a clean, working state.
Generate biosafety protocols, SOPs, records of maintenance and other records according to university guidelines.