Research Technician III

Fred Hutchinson Cancer Center•Seattle, WA

8d•Onsite

About The Position

This research opportunity is with the McGuire Laboratory, in the Vaccine and Infectious Disease Division at the Fred Hutchinson Cancer Center. Our lab is recruiting a Research Technician III to support our efforts to develop vaccines against pathogens of public health importance such as EBV, KSHV, and RSV. The technician will be expected to undertake several roles in a highly collaborative environment. Responsibilities include carrying out immunizations in small animal models and evaluating vaccine responses to different vaccine antigens using ELISA and flow-cytometry based viral neutralization assays, as well as T cell assays including intercellular cytokine staining and ELISPOT. The role may require cell line development using lentiviral transduction, confirmation of transgene expression by immunofluorescence microscopy and in vivo imaging of transferred cells using IVIS. In addition, the role will require maintaining cell cultures and general lab upkeep. The candidate will be responsible for performing and coordinating the assays with other lab members and analyzing the data. They will interpret and report on experimental results and assist with data presentation. They will assist in writing laboratory SOPs, reports, and other documentation. They will report to a Staff Scientist, the Principal Investigator and work closely with a post-doctoral scientist.

Requirements

Bachelor’s degree in related scientific field or equivalent combination of education and experience.
5 years of experience working in a lab outside of school coursework (or equivalent combination of advanced degree and work experience).

Nice To Haves

Specific experience working with mice.
Experience with serological and cell-based immune assays.
Experience with design and analysis of multicolor flow cytometry panels.
Motivation, adaptability and ability to be a strong team player are essential, as is a keen interest in vaccinology, immunology or biochemistry.
Great attention to details, well-organized, self-motivated, and able to efficiently manage time and experiments.
Willing to work with biohazardous/infectious agents (e.g., bloodborne pathogens).

Responsibilities

Maintain a high level of accuracy for all work.
Work well as team-member, participate in positive communication, and foster an environment that allows all laboratory staff to work well together.
Maintain cell cultures and produce viral stocks for conducting neutralization experiments
Conduct immunogenicity studies in mice and analyze immune responses
Proactively prepare reagents for experiments and for general lab use.
Carry out maintenance, trouble-shooting and QC of laboratory equipment.
May lead or supervise other laboratory personnel and be responsible for the daily operation of the laboratory.
Collaborate with primary investigator, and assist in designing experiments; examine proposals to determine projects and methodologies best suited to research objectives and the overall goals of the laboratory.
Summarize and write for publication or presentation the results of research projects, often as co-author or author.
Edit and write research reports.
Conduct literature reviews to assist in determining most suitable methods to be used in research.
Other duties as assigned.