Research Technician III

Duke Careers•Durham, NC

3d•$23 - $35•Onsite

About The Position

A full-time research technician position is available at the Sipkins Lab in the Dept. of Medicine at Duke University. The lab seeks to define the molecular characteristics of tissue microenvironments that foster the survival and metastasis of cancerous stem cells. In combination with molecular and cell biological approaches, the lab utilizes state-of-the-art in vivo multiphoton and confocal optical imaging techniques to explore these questions. Prior experience with immune cell assays is particularly desirable.

Requirements

Completion of a bachelor's degree in botany, biology, zoology, psychology or other science related scientific field program.
Two years of research experience. A related master's degree may offset required years of experience on a 1:1 basis, e.g., a two year master's degree in lieu of two years of experience.
General understanding of Molecular and Cell Biology concepts.
Demonstrated basic laboratory research skills, including skills in making solutions and reagents.
Attention to detail, strong oral and written communication skills, and ability to perform multiple tasks.
Good working knowledge of basic chemistry and ability to perform basic calculations as needed to prepare solutions and report data.

Nice To Haves

Prior experience with immune cell assays is particularly desirable.
Strong familiarity with Word, Excel and PowerPoint.
Must be open to working with mice.
Excellent communication and organizational skills.

Responsibilities

Assist in general laboratory maintenance and administration. This includes but is not limited to ordering, inventory, writing of standard operating procedures and supporting other lab personnel.
Mammalian tissue culture (care of cell lines, primary cells, freezing cells, transfection, clonal isolation), histology, immunohistochemistry/immunofluorescence, flow cytometry, microscopy (fluorescence, confocal), mouse work (general care, breeding, minor surgical procedures, dissection), and molecular biology procedures (RNA/DNA isolation, RT-PCR, subcloning).
Maintain accurate, up-to-date and well-documented records of procedures performed (lab notebook, instrument logs, etc) and method development.