Research Technician II - PSC Core Facility

Children's Hospital of Philadelphia•Philadelphia, PA

4d•$43,900 - $54,800•Onsite

About The Position

This entry-level research technical role participates in planned research projects by performing research protocols, experimental procedures, and other laboratory tasks under direct supervision. May occasionally exercise minimal discretion and judgment in the execution and interpretation of experiments and derived data. The Pluripotent Stem Cell (PSC) Core within the Center for Cellular and Molecular Therapeutics at The Children’s Hospital of Philadelphia (CHOP) is seeking a highly motivated Research Technician II. This position provides hands-on support for core services involving human pluripotent stem cells, including iPSC culture, differentiation workflows, and CRISPR-based gene editing, in a fast-paced, highly collaborative environment. The successful candidate will be exceptionally detail-oriented, organized, and committed to thorough documentation to support reproducibility and core operations. This role will focus on routine maintenance and expansion of PSC/iPSC lines, execution of differentiation workflows as needed, and participation in CRISPR gene editing and downstream screening/validation support. Strong note-taking, sample tracking, and organizational skills are essential.

Requirements

High School Diploma / GED - Required
At least one (1) year of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience - Required
Intermediate understanding of lab procedures, protocols, and equipment.
Basic exposure to lab specific experiments / techniques and scientific analysis
Excellent verbal and written communications skills
Excellent organizational skills
Solid critical thinking / problem-solving skills
Solid analytical skills

Nice To Haves

Associate's Degree - Preferred
Bachelor's Degree - Preferred
At least two (2) years of related classroom experience or successful completion of a technical training program (eg. Wistar, WPSI) and research laboratory experience - Preferred
neural lineage experience is a plus

Responsibilities

Maintain and expand PSC/iPSC cultures (routine culture, passaging, cryopreservation, thawing, QC support)
Perform differentiation workflows as needed (neural lineage experience is a plus)
Execute CRISPR gene editing–related tasks (e.g., preparation, delivery support, and coordination of downstream clone screening/validation workflows as appropriate for the core)
Follow established SOPs and contribute to protocol optimization when appropriate
Prepare reagents, maintain inventory, and support ordering/supply management
Maintain shared lab/core spaces, equipment logs, and general lab organization
Communicate effectively with core staff, investigators, and trainees; support core users as needed
Maintain excellent documentation: clear experimental notes, sample labeling and tracking, data organization, and accurate records in electronic and/or paper notebooks (per core requirements)
Performs routine tests / experiments under the direction of principal investigator, supervisor or other designee
Performs research procedures on experimental samples as directed
Records and checks experiments/ results for completeness and accuracy
Performs calculations and maintains appropriate log and lab notebooks
Performs tasks that require familiarity with fundamental techniques and lab equipment
May assist with planning specific research protocols and procedures
Maintains office, lab area, solutions, reagents, supplies and equipment, disposes of specimens, media and other materials (using appropriate procedures)
Assists in care and maintenance of lab equipment
May coordinate schedules of tests and procedures
Attends courses, seminars, and lab meetings to expand knowledge of research and laboratory techniques
Records results, organizes data and maintains lab notebook in an organized manner
Compiles and helps analyze data, prepares charts, graphs, and results of studies as directed
Conducts literary searches as directed