Research Technician II

Fred Hutchinson Cancer Center•Seattle, WA

1d•Onsite

About The Position

A Research Technician II position is available to work in the Collisson Lab in the Human Biology Division to focus on lung or pancreatic cancer using a combination of genetically engineered mouse models, cell lines, and clinically derived samples. The ideal candidate will be able to independently develop, guide, and complete research projects.

Requirements

Bachelor's degree in a related field
2 years of experience working in a lab outside of school coursework
Demonstrated ability to work as part of a team and independently.
Strong ability to think rationally, problem solve and troubleshoot experiments.
Excellent attention to details and the ability to work independently are essential to success in this position, as are good communication and organizational skills.
The candidate must be self-motivated, able to multitask and to work within a very collaborative environment.
Molecular biology techniques, including molecular cloning, nucleic acid isolation, PCR, and protein expression analysis.
Experience perform nucleic acid quality and quantity assessment.
Experience performing gene transfer studies using lentiviral infection of human cell cultures

Nice To Haves

Knowledge in computational processing of RNA and DNA sequencing files
Experience primary and established immortalized cell cultures
Experience establishing and maintaining mouse colonies

Responsibilities

Performing standard and investigative laboratory work in support of scientific research by performing bench-level experiments, operating analytical instruments, recovering, compiling and verifying the accuracy of research data, performing calculations, and preparing charts, graphs and summaries.
Prepare and maintain laboratory reagents, stocks, cultures or cell lines
Perform and modify documented protocols and assays
Maintain laboratory records and compile data on experiments and assays
Interpret and report on experimental results
Perform statistical analysis of test results and prepare written reports on work performed
Operate and carry out basic maintenance of laboratory equipment
Order and maintain appropriate levels of supplies
May supervise work-study students
Participate in lab meetings and journal clubs
Perform other duties as required
Prepare sequencing libraries from DNA (and, where applicable, RNA-derived cDNA) samples using established kits and protocols.
Execute fragmentation, end-repair, adapter ligation, size selection, and PCR amplification steps; perform bead-based cleanup and normalization.