UNIV - Research Specialist III - Department of Pharmacology & Immunology
About The Position
The Research Specialist III will support the experimental bone marrow transplantation laboratories and tumor immunology laboratory of Dr. Paczesny in the Hollings-NCI designated Cancer Center. The work focuses on pre-clinical models of Graft-versus-Host disease and acute myeloid leukemia, including animal observation and scoring, procurement and breeding of experimental animals, and coordination of their housing and care. The position also supports laboratory operations, regulatory compliance, and safety, working with IACUC and other agencies. The specialist will participate in transplantation procedures, including marrow isolation, spleen cell purification, irradiation and/or chemotherapy, and small-animal handling and injections. Laboratory experiments include cellular immunology methods such as CTL assays, MLR cultures, ELISAs, flow cytometry including spectral flow, cell isolation and purification, cell culture, and functional assays. The role also involves analyzing research data, maintaining protocols and records, training new lab members in basic experiments, and supporting daily laboratory and animal-care operations.
Requirements
- Bachelor's degree in chemistry, biology, or another natural, life, or health care science directly related to the assigned research area.
- Two years of advanced journey-level work experience in a clinical or research setting.
- Ability to work rotating shifts as required.
- Ability to work overtime as required.
- Ability to learn and use new processes, tools, and equipment as required.
Nice To Haves
- Master's degree and at least seven years of experience in immunology, microbiology, molecular biology, cell biology, or biochemistry.
- Ability to work independently and good management skills.
- Proficiency with Microsoft Word, PowerPoint, Excel, GraphPad, and Endnote.
Responsibilities
- Perform immunologic experiments using murine models, including harvesting organs, digesting tissues, preparing samples, and performing flow cytometry and confocal experiments.
- Isolate and purify T cells, regulatory T cells, and other hematopoietic or immune cells, and sort cells as needed.
- Perform cell isolation, cell culture, and in-vitro functional assays; maintain cell lines and perform viability, cytotoxicity, transfection, and infection assays.
- Harvest murine organs, including spleen, liver, bone marrow, and lungs, and prepare single-cell suspensions.
- Handle and monitor mice, administer treatments, and perform experimental procedures, including intravenous and intraperitoneal injections.
- Assist with mouse breeding, colony management, and genotyping of transgenic animals; maintain accurate animal records and comply with animal-care and biosafety protocols.
- Participate in transplant procedures, including marrow isolation, spleen-cell purification, irradiation and/or chemotherapy, and small-animal handling and injections.
- Collect and analyze experimental data and generate figures.
- Support daily laboratory operations by stocking consumables and reagents, preparing buffers, aliquoting reagents, and filling liquid nitrogen containers.
- Autoclave biological waste and prepare and maintain laboratory supplies and work areas, including tips, hoods, and incubators.
- Coordinate with MUSC to address laboratory maintenance, repairs, waste disposal, and cleaning needs.
- Help prepare and refine laboratory protocols and keep IBC and IACUC protocols up to date.
- Train new laboratory members in basic experiments.
- Perform additional duties assigned by the Principal Investigator.
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What This Job Offers
Job Type
Full-time
Career Level
Mid Level
