Research Scientist, Human Cell Models — Immunology & Molecular Cell Biology (Contract)

Vertex Pharmaceuticals•Boston, MA

1d•$60 - $65•Onsite

About The Position

Vertex is seeking a highly motivated Research Scientist, Human Cell Models to support immunology and molecular/cell biology research at the Boston Research site. This scientist will contribute to the development, optimization, and execution of relevant human cell models and cell-based assays to support target identification, validation, small molecule discovery screening, and mechanism-of-action studies. The ideal candidate will have strong hands-on expertise in primary cell culture, including PBMC and lymphocyte culture and differentiation, immunophenotyping, molecular biology, genome engineering, and assay development. A successful candidate will be scientifically curious, technically rigorous, and comfortable working in a fast-paced research environment to build disease-relevant models, enable translational biology, and generate actionable insights for research programs.

Requirements

Hands-on experience with PBMC isolation, enrichment and culture of primary lymphocyte populations (B cells, T cells, and other immune subsets), immune cell functional assays, and culture of primary and immortalized human cell lines.
Proven expertise in multicolor flow cytometry and immunophenotyping, including experience with complex panels.
Experience developing and executing cellular assays for protein quantification, protein interactions, localization, signaling, or reporter-based readouts using technologies such as luciferase, AlphaLISA, FRET, BRET, and/or Western blotting.
Experience with cytokine profiling platforms such as MSD and Luminex.
Ability to independently design and execute experiments, troubleshoot technical issues, interpret biological data, and manage multiple priorities.
Strong written and verbal communication skills, with the ability to present results clearly and work effectively in a collaborative team environment.

Nice To Haves

Background in stromal biology, inflammation, immune cell activation, differentiation, and/or signaling pathways.
Experience working with primary or immortalized cell models.
Familiarity with Python coding, bioinformatics, NGS analysis, or large assay data analysis.
Experience supporting interdisciplinary research programs in a fast-moving discovery environment.

Responsibilities

Design, execute, analyze, and interpret cell-based and immunology-focused experiments to support target identification, target validation, small molecule discovery, and mechanism-of-action studies.
Develop, optimize, and troubleshoot in vitro human cell model systems and assay workflows using immune, primary, and/or immortalized cell models.
Culture, maintain, and evaluate B cells, PBMC-derived immune cell populations, primary human cells, and/or immortalized cell lines for functional and mechanistic studies.
Perform and optimize immunophenotyping assays using multicolor flow cytometry, including complex panel design and data interpretation.
Develop and miniaturize cell-based assays, including fluorescent, luminescent, FRET/BRET, luciferase, AlphaLISA, Western blot, and other protein quantification or localization assays for screening and mechanistic applications.
Routinely execute developed cell-based assays to support medicinal chemistry design cycles.
Perform cytokine profiling and immune functional readouts using platforms such as MSD and Luminex.
Analyze datasets, interpret biological results, and clearly communicate findings, recommendations, and next steps to project teams.
Maintain accurate, timely, and high-quality experimental records and documentation aligned with Vertex standards.
Collaborate closely with cross-functional scientists and interdisciplinary project teams to support program goals, scientific decision-making, assay improvements, data quality, and reproducibility.