Scientist in Protein Technologies Center - Cell Engineering / Molecular Biology
About The Position
The Protein Technologies Center (PTC), within the Department of Structural Biology, is seeking a scientist skilled in saturation-mutagenesis-reinforced functional assays, site-specific integrase-based stable expression platforms, and genome-editing technologies. The scientist will systematically tag endogenous proteins with fluorescent and affinity tags, enabling structural, functional, and drug-discovery studies, and supporting investigations of protein interactions and localization. The PTC is a multidisciplinary team spanning protein engineering, cloning, expression, purification, biophysical analysis, and structure determination. Cryo-electron microscopy has given us a window into the molecular and atomistic details of the cell, and molecular engineering of living cells is how we get there. This is a bench-based role: the scientist will edit cell lines, confirm that editing was successful, and verify that genomic stability is maintained throughout the clonal line-generation process — providing pivotal, hands-on support to structural cell biology projects across the department, both strategically and tactically.
Requirements
- Proven track record in molecular cloning (In-Fusion or LIC or PIPE or Gateway or FX; overlap extension PCR/Gibson assembly/BioBrick/recombineering), handling large and/or complex DNA constructs, including combinatorial libraries; site-directed and/or saturation mutagenesis
- Bachelor's degree in relevant scientific area is required.
- Eight (8) years of relevant work experience is required with a Bachelor's degree.
- Six (6) years of relevant work experience is required with a Master's degree.
- Three (3) years of relevant work experience is required with a PhD.
Nice To Haves
- PhD (with at least 5 years of postdoctoral experience) in molecular biology or synthetic biology, or any related discipline preferred.
Responsibilities
- Engineer cell lines using CRISPR gene editing technology for generating gene knockouts and knock-ins (SNVs, gene tags, etc.) and confirm the edits.
- Generate stable cell lines using non-viral and viral-based delivery systems.
- Implement advanced technologies to develop stable target-overexpressed cell lines, pools, clones, and NGS library preparation.
Benefits
- Medical & Prescription Drug Plans
- Dental Plan
- Vision Plan
- Financial Security
- Retirement Plan
- Living Well - Employee Wellness Program
- Time Off Programs
- Educational Benefits
- Family Friendly Benefits
- Benefits Unique to St. Jude
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What This Job Offers
Job Type
Full-time
Career Level
Senior
