Research Associate/Senior Research Associate, Genome Engineering

Colossal•Dallas, TX

1d•Onsite

About The Position

An affiliate of Colossal is seeking an experienced Research Associate/Senior Research Associate to support precision genome engineering in non-model mammalian organisms. The role produces and validates CRISPR/Cas9, prime editing, base editing, and multiplex editing reagents that enable Cell Engineering colleagues to generate edited cell lines. The position is for a technically proficient, hands-on contributor who can independently execute established workflows and contribute from the outset. It is based on-site in Dallas, Texas, and relocation assistance is available. The role works with Genome Engineering scientists to implement and troubleshoot editing designs, then transfers validated reagents and clear experimental documentation to Cell Engineering colleagues for transfection and downstream cellular workflows. It also supports PCR-based genotyping, sequencing preparation, and analysis of single-locus and multiplex editing outcomes while maintaining laboratory records, inventories, and quality-control documentation. Colossal describes its culture as positive, hopeful, collaborative, creative, and committed to making a difference. The company welcomes people from all backgrounds and is committed to an inclusive workplace free from discrimination and harassment.

Requirements

BA/BS or MA/MS in molecular biology, genetics, biotechnology, bioengineering, or a related field.
Demonstrated hands-on molecular biology research experience, including cloning, PCR, plasmid preparation, and sequence verification.
Practical experience supporting CRISPR/Cas9 genome editing and multiplex editing experiments or reagent workflows.
Experience preparing, handling, and quality-controlling DNA and/or RNA editing reagents.
Ability to independently execute established protocols, troubleshoot routine technical problems, and contribute immediately with limited training.
Strong organizational skills, attention to detail, and written and oral communication skills.

Nice To Haves

Hands-on experience with prime editing and/or base editing reagents and workflows.
Experience designing or preparing pegRNAs, modified guide RNAs, or HDR donors.
Experience with in vitro transcription, RNA purification, or nucleic acid QC.
Experience with sequencing-based genotyping and analysis of multiplex edits.
Experience with electronic laboratory notebooks and cross-functional reagent handoffs.

Responsibilities

Generate and quality-control genome editing reagents, including sgRNAs, pegRNAs, donor DNA templates, plasmids, and expression constructs.
Perform molecular cloning, DNA assembly, bacterial culture, plasmid purification, PCR, and sequence verification.
Support preparation and quality control of RNA reagents, including in vitro transcription and purification where applicable.
Prepare and track reagent sets for multiplex genome editing experiments, maintaining accurate construct and sample identities.
Work with Genome Engineering scientists to implement and troubleshoot CRISPR/Cas9, prime editing, and base editing designs.
Transfer validated reagents and clear experimental documentation to Cell Engineering colleagues for transfection and downstream cellular workflows.
Perform PCR-based genotyping, prepare samples for sequencing, and support analysis of single-locus and multiplex editing outcomes.
Maintain accurate electronic laboratory records, reagent inventories, and quality-control documentation in ELN.
Organize concurrent experiments, communicate results, and proactively improve workflows.