Research Associate/Senior Research Associate, Genome Engineering

Colossal Biosciences•Dallas, TX

1d•Onsite

About The Position

An affiliate of Colossal is seeking an experienced Research Associate/Senior Research Associate to support precision genome engineering in non-model mammalian organisms. You will produce and validate CRISPR/Cas9, prime editing, base editing, and multiplex editing reagents that enable Cell Engineering colleagues to generate edited cell lines. We seek a technically proficient, hands-on contributor who can execute established workflows independently and contribute from the outset. This position is based on-site in Dallas, TX. Relocation assistance is available.

Requirements

BA/BS or MA/MS in molecular biology, genetics, biotechnology, bioengineering, or a related field
Demonstrated hands-on molecular biology research experience, including cloning, PCR, plasmid preparation, and sequence verification
Practical experience supporting CRISPR/Cas9 genome editing and multiplex editing experiments or reagent workflows
Experience preparing, handling, and quality-controlling DNA and/or RNA editing reagents
Ability to independently execute established protocols, troubleshoot routine technical problems, and contribute immediately with limited training
Strong organizational skills, attention to detail, and written and oral communication skills

Nice To Haves

Hands-on experience with prime editing and/or base editing reagents and workflows
Experience designing or preparing pegRNAs, modified guide RNAs, or HDR donors
Experience with in vitro transcription, RNA purification, or nucleic acid QC
Experience with sequencing-based genotyping and analysis of multiplex edits
Experience with electronic laboratory notebooks and cross-functional reagent handoffs

Responsibilities

Generate and quality-control genome editing reagents, including sgRNAs, pegRNAs, donor DNA templates, plasmids, and expression constructs
Perform molecular cloning, DNA assembly, bacterial culture, plasmid purification, PCR, and sequence verification
Support preparation and quality control of RNA reagents, including in vitro transcription and purification where applicable
Prepare and track reagent sets for multiplex genome editing experiments, maintaining accurate construct and sample identities
Work with Genome Engineering scientists to implement and troubleshoot CRISPR/Cas9, prime editing, and base editing designs
Transfer validated reagents and clear experimental documentation to Cell Engineering colleagues for transfection and downstream cellular workflows
Perform PCR-based genotyping, prepare samples for sequencing, and support analysis of single-locus and multiplex editing outcomes
Maintain accurate electronic laboratory records, reagent inventories, and quality-control documentation in ELN
Organize concurrent experiments, communicate results, and proactively improve workflows