Research Associate, In Vivo

Flagship Pioneering, Inc.•Cambridge, MA

6d•$65,000 - $93,500•Onsite

About The Position

We are seeking a highly motivated Research Associate with broad expertise conducting in vivo studies, histology preparation and analysis, and molecular biology. This individual will play an important role in generating high-quality data on the biodistribution, target engagement, and biological response of our molecules in animal models. The candidate must be independent, goal-oriented, have excellent communication skills, and be willing to work in a highly dynamic and fast-paced entrepreneurial environment. This is a unique opportunity to drive the growth of an early-stage biotechnology company where you can contribute to the next frontier of genetic medicines. We are looking for collaborative, enthusiastic problem-solvers to join our team, driven by a shared goal of making a practical impact.

Requirements

Bachelor’s degree or higher in Cell Biology or a related field, with 1+ years of laboratory experience, preferably in the biopharmaceutical industry.
Strong hands-on experience with rodent in vivo studies
Strong hands-on experience in histology, including tissue embedding, sectioning, staining, IHC/IF, and imaging.

Nice To Haves

Authorship on a peer reviewed publication
Experience with multiplex IHC/IF and advanced image analysis platforms.

Responsibilities

Conduct in vivo studies in rodent models including experience with peripheral administration (IV, IP, SC) and stereotactic surgeries for direct to CNS administration.
Conduct terminal procedures, including animal takedowns, blood collection, and necropsies.
Perform tissue harvesting and dissection as appropriate for downstream bioanalytical and histological applications.
Prepare, embed, and section a variety of mammalian tissues using microtome, vibratome, or cryostat techniques (FFPE or fresh-frozen samples).
Perform immunohistochemistry (IHC) and immunofluorescence (IF) on tissue sections
Acquire high-quality images using epifluorescence and/or confocal microscopy
Perform in vivo and ex vivo fluorescence and bioluminescence imaging using IVIS or equivalent small-animal imaging systems, including organ collection, image acquisition, and quantitative analysis of signal intensity and tissue distribution.
Conduct quantitative image analysis (cell counts, regional MFI, colocalization analysis, morphometric quantification) using software such as ImageJ, QuPath, Halo, or equivalent platforms.
Perform measurement of gene and protein expression analyses using qRT-PCR, Western blotting, and ELISA methodologies and interpret data
Report results clearly to the scientific team