Research Assistant I NEUROLOGY kl
About The Position
The Research Assistant I works independently under the supervision of Vikram Khurana, MD, PhD, and a senior researcher in the Khurana lab. Depending on project needs and the candidate’s skills, the role may involve molecular biology bench work and development or optimization of viability and toxicity assays in neurons derived from induced pluripotent stem cells (iPSCs). Methods include flow cytometry, longitudinal neuron imaging, plate-based live-cell and calorimetric assays, and western blotting. The position supports the Principal Investigator and study team through routine experiments using established methods, data collection and management, and other assigned research activities. The candidate is expected to be diligent, highly organized, and collegial. The position also supports epidemiological and other population-based research, including study cohort management and communication with subjects. The role is onsite at The Brigham and Women's Hospital, Inc., with 40 scheduled hours per week on the day shift. Patient care is not required.
Requirements
- Bachelor's degree in science required; experience may be accepted in lieu of a degree.
- Excellent cell culture practices and sterile technique.
- Medium to high computer literacy and a basic understanding of statistical concepts.
- Excellent oral and written communication skills.
- Strong interpersonal skills for working in a diverse lab.
- Excellent organizational skills and ability to prioritize a variety of tasks.
- Excellent data management skills and attention to detail.
- Computer literacy, including database tools.
- Ability to follow directions and demonstrate professionalism.
Nice To Haves
- 0–1 year of experience preferred; suitable for a new graduate with relevant coursework.
- Experience with human cell culture, such as HEK293 or another immortalized cell line.
- iPSC stem cell culture experience.
- Basic understanding of molecular techniques, microscopy, and flow cytometry.
- Understanding of molecular cloning, western blotting, RNA isolation, and qPCR.
- Good presentation skills.
Responsibilities
- Maintain, passage, catalogue, and culture iPSC cells.
- Differentiate iPSCs into neurons and glia and age these cultures.
- Maintain the Attune flow cytometer and order related reagents.
- Measure and schedule longitudinal imaging using the Incucyte and Nikon Biostation microscope.
- Maintain HEK293 cells and generate stable cell lines.
- Assist with molecular cloning, including Gibson assembly, Gateway cloning, and mini/maxi preps.
- Analyze data from western blotting, microscopy, and flow cytometry.
- Maintain detailed lab notes, primarily digitally using LabArchives, and generate standard operating procedures for the lab.
- Organize and label experiments and manage files.
- Report daily to the immediate supervisor and weekly to the Principal Investigator.
- Work with junior and senior lab members to troubleshoot experiments.
- Share laboratory duties with other technicians.
- Collect, manage, manipulate, analyze, and report data.
- Manage study cohorts.
- Communicate with subjects, conduct mailings, follow up on mailings, and obtain necessary documents.
- Assist with epidemiological studies and other population-based research.
Benefits
- Comprehensive benefits.
- Career advancement opportunities.
- Differentials, premiums, and bonuses as applicable.
- Recognition programs supporting professional growth.
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What This Job Offers
Job Type
Full-time
Career Level
Entry Level
