Postdoctoral Research Fellow - Pavani Lab

Children's Hospital of Philadelphia•Philadelphia, PA

4d•Onsite

About The Position

The Pavani Lab at The Children’s Hospital of Philadelphia (CHOP) seeks a motivated postdoctoral fellow to join a collaborative, multidisciplinary team focused on genome editing and red cell engineering for translational research. The successful candidate will lead projects that apply CRISPR/Cas9 and other genome engineering approaches in mammalian cell systems, with the goal of advancing novel therapeutic strategies and improve blood transfusions.

Requirements

PhD in Molecular Biology, Genetics, Cell Biology, Bioengineering, or a related field
Demonstrated hands-on experience with genome editing techniques (CRISPR/Cas or similar).
Strong experience in mammalian cell culture and experimental manipulation.
Practical experience with flow cytometry (sample prep, acquisition, and analysis).
Ability to design experiments, analyze data quantitatively, and communicate findings clearly.

Nice To Haves

Experience with next-generation sequencing workflows relevant to genome editing (amplicon-seq, targeted sequencing, library prep) and basic bioinformatic analysis of NGS data.
Previous work with induced pluripotent stem cells (iPSC) — culture, maintenance, differentiation, or genetic manipulation.
Familiarity with erythropoiesis and flow cytometry
Experience in vector design, and safe handling of viral vectors (Lenti).
Track record of publications in the genome engineering or cell therapy fields.
Strong organizational skills, ability to work collaboratively across diverse teams, and mentoring experience with junior trainees

Responsibilities

Design, optimize, and execute genome-editing experiments (CRISPR/Cas systems, base editors, prime editors, or equivalent) in mammalian cells.
Develop and maintain cell cultures, including induced pluripotent stem cells (iPSCs) and immortalized lines.
Perform genome engineering workflows: vector design and cloning, transfection/electroporation, editing efficiency and genotyping assays.
Characterize edited cells using flow cytometry (including sorting), immunophenotyping, and functional assays.
Prepare next-generation sequencing (NGS) assays for on-/off-target analysis and deep sequencing of edited loci; analyze sequencing data.
Troubleshoot experiments, optimize protocols, and maintain rigorous documentation and lab records.
Present data at internal meetings and conferences; contribute to manuscript and grant preparation.