Postdoctoral Research Associate, Department of Surgery

UVA Health • Charlottesville, VA

112d • Onsite

About The Position

The Brayman Lab in the Department of Surgery at the University of Virginia is seeking a Postdoctoral Research Associate to perform and assist with studies investigating the underlying mechanisms of type 1 diabetes and recurrence of diabetes in various murine models of diabetes. The position will also support human pancreatic islet isolations in the GMP islet isolation facility. Postdoctoral employment is temporary and is normally limited to an individual who has been awarded a Ph.D. or equivalent doctorate within the previous five years and who will be involved in full-time research or scholarship at the University. Employment as a Postdoctoral Research Associate is viewed as training and is preparatory for a full-time academic or research career, is supervised by a senior scholar, and allows the appointee to publish the results of his/her research or scholarship during the training period.

Requirements

• PhD or MD in Biology, Chemistry, Cell Biology, Molecular Biology, Biochemistry, Biomedical Engineering, or a related field
• Enthusiastic team-oriented individual with a willingness to learn, collaborate, and take on new challenges
• Ability to work a flexible schedule when required

Nice To Haves

• Experience working actively in a research laboratory
• Experience in standard cell biology techniques and murine work
• Flow cytometry and CyTOF
• Protein purification (including immunoglobulins such as IgM) and chromatography
• Experience with Akta go FPLC system
• Proteomics
• Experience with mass spectroscopy (LC-MS), glycan preparation, data analysis
• Immunoassays, including ELISAs
• Electrophoresis and Western blot
• PCR, RT-PCR
• DNA and RNA extractions for NGS, RNAseq/ scRNAseq/ cfRNAseq
• Tissue culture / in vitro cell culture
• Histology analysis and immunostaining of tissue sections to back up imaging mass cytometry
• Immunohistochemistry, Immunofluorescence, Immunocryohistochemistry
• Stem cells and organoids
• Imaging mass cytometry
• Confocal
• Experience in R and Python, with additional experience in downstream pipeline analysis and additional expertise in statistical and data analysis software

Responsibilities

• Working with mice and performing/assisting with in vitro and in vivo experiments related to ongoing studies
• Working with murine models of diabetes, including mouse islet isolation and transplantation
• Surgical procedures
• Isolating islets
• Measuring mouse blood glucose levels
• Collection of mouse tissue, serum, and blood, including collection of mouse tissue and blood for proteomic studies
• Assistance with collection and entry of data in electronic media
• Supporting daily lab activities, maintenance, and general laboratory upkeep
• Ordering/purchasing materials and supplies, including purchasing through the procurement system
• Other duties as assigned
• Perform routine laboratory data analysis and research procedures
• Independently prepare solutions and reagents
• Perform or learn to work with animal models (including surgical techniques; training provided if necessary)
• Work diligently with strong attention to detail
• Analyze and organize work procedures; plan and execute tasks/experiments; interpret and report findings, writing skills
• Train in and comply with BSL-2 procedures
• Demonstrate reproducibility and strong critical thinking skills

Benefits

• Benefited position
• UVA benefits

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Education Level: Ph.D. or professional degree