Post-Doctoral Fellow

Jefferson Health Plans • Philadelphia, PA

2d • Onsite

About The Position

This role involves acquiring proficiency in 3D electron microscopy (EM) specimen handling and preparation for data acquisition, as well as setting up data acquisition using transmission electron microscope (TEM) and focused ion beam scanning electron microscope (FIB-SEM). The position requires understanding sample behavior during acquisition and identifying sources of errors/noises that affect post-acquisition 3D reconstruction and analysis. The fellow will implement and develop software codes for post-acquisition image stack optimization, distortion corrections, and stack alignment. Additionally, they will implement and develop AI/machine learning workflows for 3D reconstructions, automated image segmentations, and 3D ultrastructural and geometric analyses. The role also includes coordinating and supervising the use of computers in the FIB-SEM lab for 3D image analysis. The fellow will contribute to 3D-EM projects focused on morphometric/geometric analysis of mitochondrial networks, membrane formations, and organelle encounters in various tissue types. Communication of methodological advances at conferences and through peer-reviewed publications, as well as managing data sharing and code availability, are key aspects of this position.

Requirements

• Proficiency in 3D electron microscopy (EM) specimen handling and preparation.
• Experience with data acquisition using transmission electron microscope (TEM, tilt-angle series).
• Experience with focused ion beam scanning electron microscope (FIB-SEM, slice-and-view workflow).
• Understanding of sample behavior during acquisition and sources of acquisition errors/noises.
• Ability to implement and develop software codes for image stack optimization, distortion corrections, and stack alignment.
• Ability to implement and develop AI/machine learning workflows for 3D reconstructions, automated image segmentations, and 3D ultrastructural and geometric analyses.
• Experience in coordinating and supervising the use of lab computers for 3D image analysis.
• Contribution to 3D-EM projects focusing on morphometric/geometric analysis.
• Communication of methodological advances at conferences and peer-reviewed scientific publications.
• Management of data sharing and code availability.

Responsibilities

• Acquire proficiency in 3D electron microscopy (EM) specimen handling and preparation for data acquisition.
• Set up data acquisition using transmission electron microscope (TEM, tilt-angle series) and focused ion beam scanning electron microscope (FIB-SEM, slice-and-view workflow).
• Understand sample behavior during acquisition and sources of acquisition errors/noises.
• Implement and develop software codes for post-acquisition image stack optimization, distortion corrections, and stack alignment.
• Implement and develop AI/machine learning workflows for 3D reconstructions, automated image segmentations, and 3D ultrastructural and geometric analyses.
• Coordinate and supervise the use of computers of the FIB-SEM lab dedicated to 3D image analysis.
• Contribute to 3D-EM projects focusing on the morphometric/geometric analysis of mitochondrial networks, membrane formations and organelle encounters.
• Communicate methodological advances at conferences and peer-reviewed scientific publication platforms.
• Manage data sharing and code availability.

Benefits

• Medical (including prescription) insurance
• Supplemental insurance
• Dental insurance
• Vision insurance
• Life and AD&D insurance
• Short- and long-term disability
• Flexible spending accounts
• Retirement plans
• Tuition assistance
• Voluntary benefits (group rates on insurance and discounts)
• Tuition discounts at Thomas Jefferson University (after one year of full-time service or two years of part-time service)

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Education Level: Ph.D. or professional degree