Laboratory Technician

Marshall UniversityHuntington, WV
Onsite

About The Position

The Xu Lab, in the Department of Biomedical Sciences at Marshall University’s Joan C. Edwards School of Medicine, is seeking a part-time technician. The primary objective of this position is to construct genetic mutants of Streptococcus pyogenes.

Requirements

  • PCR
  • Restriction cloning
  • Plasmid purification
  • Gel electrophoresis
  • Bacterial transformation (electroporation or chemical competence)
  • Screening PCR
  • DNA sequencing data analysis

Responsibilities

  • Design and generate targeted gene mutations in streptococcal strains.
  • Perform PCR, restriction cloning, plasmid purification, and gel electrophoresis.
  • Introduce recombinant DNA into bacteria using electroporation or chemical competence.
  • Validate mutant strains using screening PCR and DNA sequencing data analysis.
  • Maintain clean records of primers, plasmids, and newly created glycerol stocks.
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