Cell Culture Specialist I

MESO SCALE DIAGNOSTICS, LLC.•Gaithersburg, MD

2d•$59,100 - $87,900•Onsite

About The Position

Under the direction of the manager, this position is responsible for the production of cell-based products including but not limited to: hybridoma antibodies and recombinant antibodies. Primarily responsible for cell culture and managing multiple purifications systems.

Requirements

Bachelor's degree in Biology, Chemistry, Biotechnology, Engineering or a related field or equivalent applicable work experience. May substitute degree for equivalent combination of experience/education.
Minimum of three months of experience performing cell culture using aseptic technique in a laboratory setting required.
Experience in a GMP/GLP, ISO Certified environment preferred.

Nice To Haves

Knowledge of manufacturing mammalian cell based products preferred
Knowledge of Hybridoma cell culture and expansion preferred.
Knowledge of common cell culture equipment: Hemocytometer, Biological Safety Cabinet, Automated Cell Counter, Plate Readers (for ELISA and BCA assays), LN2 Freezers, Humidified Incubators, Water Baths, CO2 Tanks, and Controlled Rate Freezer.
Proficiency in MS Office Suite and SharePoint preferred.

Responsibilities

Manipulate multiple cell lines (both adherent and suspension), such as mouse hybridoma cell , Expi 293 and Expi CHO cells for the production of monoclonal antibodies and other cell based products.
Execute transfections for the production of recombinant QRM antibodies.
Use the following cell culture equipment: Hemocytometer, Biological Safety Cabinet, Automated Cell Counter, Plate Readers (for ELISA and BCA assays), LN2 Freezers, Humidified Incubators, Water Baths, CO2 Tanks, and Controlled Rate Freezer.
Use AKTA purification systems
Provide general cell culture laboratory maintenance. Keep process areas stocked with essential equipment and supplies and maintain overall laboratory appearance and cleanliness.
Coordinate regular equipment maintenance and calibrations; complete and document equipment validation as required in MFG.
Perform daily scheduled cell culture and purification processes to meet production demand
Perform vialing and storage of cell based products.
Maintain and organize inventory of supplies, including but not limited to cell stocks and records, to support daily production.
Other duties as assigned.