Single-Cell / Spatial Profiling Specialist

US Tech Solutions • North Chicago, IL

9d

About The Position

The Genomic Research Centre (GRC) is a unified organization dedicated to supporting and advancing genomics and genetics research across R&D. It encompasses core capabilities and research initiatives in Genomic Technologies, Functional Genomics, Computational Genomics, Human Disease Genetics, and Pharmacogenetics, with dedicated IT infrastructure support. The GRC's primary mission is to leverage genomics and genetics research for target discovery, target validation, translational research, and the design and analysis of clinical trials. The GRC Genomic Technologies Group (GTECH) aids preclinical and clinical research teams by employing genomic profiling technologies to enhance the success rates of drug discovery and development. The GRC is actively seeking a scientist skilled in Next-Generation-Sequencing (NGS) to contribute to its single-cell and Visium spatial transcriptomic profiling capabilities.

Requirements

• Hands-on single cell sequencing experience
• Hands-on spatial transcriptomics experience
• NGS data QC experience
• Hands-on NGS experience
• Typically, 7 years of experience, master’s degree, or equivalent education and typically 5 years of experience in molecular biology, genetics, genomics or a related field.
• Demonstrated record of high-proficiency, hands-on molecular biology experience with PCR-based assays and technical knowledge of high-throughput genomic profiling technologies with emphasis on Illumina sequencing platforms and complex library preparation workflows for bulkRNA-seq, 10X Genomics scRNA-seq, and 10X Genomics Visium spatial transcriptomics.
• Knowledge of QC metrics, interpretation , and demonstrated use of approaches for assessing the quality of samples and libraries for bulkRNA, scRNA, and spatial profiling, such as capillary electrophoresis, qPCR, fluorometry, spectrophotometry, cell counting and viability.
• Proficiency with basic bioinformatics tools and sequencing data analysis concepts, such as Illumina SAV, bcl2fastq/BCLConvert, fastQC, and working in a UNIX HPC environment.
• Bachelor’s Degree or equivalent education

Nice To Haves

• Experience with 10X Genomics Cellranger and/or Spaceranger software is highly preferred.
• Experience being part of a centralized high-throughput genomic lab is highly desirable.
• Proven direct end-to-end hands-on experience with 10X Genomics technologies such as single-cell RNAseq or Visium is highly preferred.

Responsibilities

• Reporting to the Single Cell Lead, work closely with other NGS Technical Leads and staff to fulfill single cell/spatial transcriptomic profiling needs for active studies from client Genomic Research Centre (GRC) stakeholders and client therapeutic area (TA) scientists.
• Support single cell and spatial transcriptomic workflows.
• Perform sample and library prep, and sequencing, initial data QC and first pass analysis of results.
• Closely work with NGS technical leads and partner with bioinformatics specialists and disease area scientists to interpret results and troubleshooting.
• Operate and maintain NGS and its supportive platforms within centralized technology group.
• Prepare written summaries of experimental results and verbally present at team meetings.
• Maintain accurate documentation of experiments, protocols, and results.
• Support inventory and maintenance.
• Effectively communicate with stakeholders, especially around experimental design, troubleshooting, and responding to changes in priorities and adjusting timelines.

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level