Senior Research Associate

Lawrence Berkeley National LaboratoryBerkeley, CA
Onsite

About The Position

Lawrence Berkeley National Laboratory has an opening for a Senior Research Associate in their Joint Genome Institute. This is a full-time, career appointment, exempt (monthly paid) from overtime pay. Work will be performed onsite at Lawrence Berkeley National Lab, located at 1 Cyclotron Road, Berkeley, CA 94720. Candidates must be eligible to work in the U.S. at the time of hire. Visa sponsorship is not available for this position.

Requirements

  • Bachelor’s Degree in Biology, Molecular Biology, Plant Biology or related field and a minimum of 2 years of relevant experience.
  • Experience with DNA/RNA purification and Next Gen Sequencing (NGS) library preparation.
  • Experience with plant growth and sampling for non-model species.
  • Experience with single-cell isolation from plant tissues.
  • Demonstrated confocal or fluorescence microscopy skills, including tissue sectioning for RNA in-situ or histology.
  • Experience with plasmid construction, cloning, and bacterial transformation.
  • Experience with sterile technique.
  • Demonstrated data analytics skills, including image processing with R, Python, and ImageJ.

Responsibilities

  • Perform experimental manipulation and sample collection for a set of advanced molecular assays.
  • Perform single-cell profiling of plant tissues undergoing environmental stress, RNA in situ hybridization experiments, confocal microscopy experiments, bacterial construct preparation and cloning, as well as bacterial growth and plant colonization profiling.
  • Prepare seeds for growth on sterile media and soil and maintain plant growth systems.
  • Prepare molecular biology buffers and reagents.
  • Prepare, analyze, and sort plant cells or nuclei.
  • Prepare, embed, section, and stain plant specimens.
  • Perform in situ hybridization experiments.
  • Image tissues using confocal and widefield microscopy.
  • Perform plant/microbe bioassays.
  • Culture, transform, and maintain bacterial strains (including rhizobacteria).
  • Accurately and thoroughly document experimental procedures and results.
  • Perform preliminary data analysis and quality control on the data collected.

Benefits

  • The foundation of Berkeley Lab is our Stewardship Values: Team Science, Service, Trust, Innovation, and Respect; and we strive to build community with these shared values and commitments.
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