Senior Research Associate - Molecular Biology
About The Position
Monod Bio is a pioneer company in the design of de novo protein reagents for the life sciences and diagnostics industries. Our innovative design platform technology leverages recent advances in deep learning computational protein engineering to create rapid, highly sensitive protein-based reagents and assays. Our visionary founding team and state-of-the-art technology and product development experience position Monod Bio at the forefront of molecular reporter, binder and functional protein innovation. We welcome exceptional individuals to join us in this journey. Monod Bio is seeking a Sr. Research Associate / Associate Scientist in Molecular Biology (depending on experience) with a strong foundation in molecular biology and protein biochemistry. In this role, you will support our Discovery Team in protein design and protein optimization efforts through advanced DNA assembly and protein mutagenesis schemes, FACS screening of yeast display libraries, and biochemical techniques for lead identification. The ideal candidate thrives in a fast-paced environment while maintaining clear, rigorous documentation.
Requirements
- A bachelor’s degree in biochemistry, biophysics, analytical chemistry, biology, biotechnology, chemical engineering, or related fields.
- 2+ years of experience with molecular biology techniques (DNA work, PCR, cloning and plasmid purification).
- Experience with staining panel development, flow cytometry, and sorting in yeast.
- Extensive experience with tracking data and recording results in electronic lab notebook format (e.g., Benchling).
- Highly organized and detail-oriented, with strong communication skills and the ability to work independently.
Nice To Haves
- 2+ years of relevant experience in life sciences in either industry or academia.
- Hands-on experience with DNA library construction and preparation for sequencing.
- Experience with yeast transformation and yeast surface display methodologies.
- Experience with biochemical and biophysical methods such as BLI, FPLC, SDS-PAGE, and SEC-MALS.
- Proven track record with protein sciences, purification, and analysis in academia or industry.
Responsibilities
- Independently perform molecular biology and protein manufacturing tasks.
- Design and conduct mutagenesis experiments to create diverse DNA libraries.
- Design and implement staining panels for FACS screening of yeast display libraries.
- Assist in experimental planning and troubleshooting under the guidance of senior scientists.
- Plan and execute standard molecular biology techniques including PCR, Golden Gate cloning, plasmid preps, and DNA purification.
- Conduct high-throughput protein expression screening in E. coli using 96-well plate formats.
- Perform binding assays using Bio-Layer Interferometry (BLI).
- Purify proteins using affinity chromatography (Ni-NTA, Strep-Tactin) and size exclusion chromatography (SEC).
- Characterize protein quality and stability using SEC-MALS, circular dichroism, and thermostability assays.
- Maintain accurate records of experiments and materials in Benchling.
- Collaborate with the team to fulfill protein production requests and ensure timely delivery.
- Support additional lab operations and projects as needed.
Benefits
- 100% company-paid medical, dental, life/AD&D, and vision insurance premiums for employees scheduled to work 25+ hours per week.
- 50% paid premiums for eligible dependents.
- 4 weeks PTO per year
- 11 paid holidays.
- Parental leave benefit.
- State-of-the-art lab and office on bus line and near coffee shops, restaurants, and South Lake Union neighborhood.
- Casual environment, friendly colleagues, and of course, excellent snacks and coffee in the kitchen!
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What This Job Offers
Job Type
Full-time
Career Level
Senior
