Scientist, Protein Expression

Anthology•Cambridge, MA

11h•Onsite

About The Position

We are looking for a hands-on scientist to drive recombinant protein expression in filamentous fungi. Reporting to the Director of R&D, you will own the bench work that sits at the core of our platform, from cloning and fungal transformation through expression screening and assay development. You will also collaborate closely with our microfluidics team to push protein screening into high-throughput microdroplet formats. This is a role for someone who is equally comfortable designing experiments and troubleshooting them at the bench.

Requirements

PhD in bioengineering, microbiology, biochemistry, or a related field
Strong molecular cloning skills and experience constructing complex genetic assemblies
Experience with protein expression and characterization including SDS-PAGE, western blot, ELISA, or equivalent assays
Able to work on-site in Cambridge, MA
Authorized to work in the United States (STEM-OPT or J1 are welcomed)

Nice To Haves

Direct experience culturing and/or engineering filamentous fungi (Aspergillus, Trichoderma, or similar)
Familiarity with protein design using biological language models (ESM, ProteinMPNN, RFdiffusion, or similar)
Python scripting experience — data processing, assay analysis, or bioinformatics workflows

Responsibilities

Design and execute molecular cloning workflows to construct and optimize fungal expression vectors
Perform fungal transformation and develop efficient DNA delivery protocols in target strains
Develop and run protein expression assays in shake flask format, from culture setup through quantification and characterization
Work with the microfluidics team to adapt and validate protein assays for microdroplet-based high-throughput screening
Mine and characterize genetic parts (e.g. signal peptides) to expand our engineering toolkit
Maintain rigorous experimental documentation to support reproducibility and platform iteration