Scientist/Sr. Scientist, Protein Purification
About The Position
As a Scientist or Senior Scientist in Protein Purification and Analysis, you will play an important scientific role supporting discovery programs, with a primary emphasis on high-quality purification of protein-based therapeutics and their bioconjugates. You will work hands-on with a broad range of modern protein purification technologies, with complementary analytical characterization, at the inception of our new Cambridge, MA research center. This role suits a scientist with deep, hands-on expertise in protein and bioconjugate purification, scientific curiosity, and a desire to grow in scope and responsibility.
Requirements
- PhD in Analytical Chemistry, Protein Engineering, Biochemistry, or a related field preferred (postdoctoral training preferred); candidates with a BS/MS and significantly more relevant industry experience will also be considered
- 2+ years of relevant post-degree experience in protein purification (Scientist); 4+ years (Senior Scientist)
- Deep, hands-on expertise in protein and peptide purification, including SEC, IEX, affinity, RP-HPLC, and CE-based methods, at analytical and preparative scales
- Working knowledge of analytical characterization techniques, including mass spectrometry, biophysical methods (e.g., DLS, MALS, SPR, BLI, DSF), and flow cytometry, sufficient to collaborate effectively with analytical specialists
Nice To Haves
- Experience with complex molecular formats such as ADCs, AOCs, or other bioconjugates strongly preferred
- Hands-on experience with AKTA chromatography systems (e.g., AKTA pure, AKTA avant) is desired
Responsibilities
- Serve as a primary supplier of high-purity protein and bioconjugate samples formatted to meet the requirements of downstream mass spectrometry (e.g., native MS, HDX-MS) and structural biology methods (e.g., cryo-EM, X-ray crystallography)
- Execute and support purification of recombinant proteins, peptides, and their bioconjugates (e.g., ADCs, AOCs) to support discovery programs from early research through lead-series selection
- Apply a broad range of purification techniques, including SEC, IEX, affinity, RP-HPLC, and CE-based methods, at analytical and preparative scales
- Support purification and characterization of challenging targets and formats, such as membrane proteins (e.g., GPCRs), in vitro-reconstituted target complexes, and peptide-Fc or lipidated conjugates
- Support analytical characterization to confirm identity, purity, concentration, and stability using mass spectrometry, chromatographic, biophysical, and flow cytometry-based methods
- Contribute to the development and optimization of automated and high-throughput purification workflows, improving speed, reproducibility, and data quality
Benefits
- Salary Range: $125,000 - $170,000
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What This Job Offers
Job Type
Full-time
Career Level
Entry Level
Education Level
Ph.D. or professional degree
