Scientist II

AbbVie•North Chicago, IL

3d•$84,500 - $162,000•Onsite

About The Position

We are seeking a highly motivated Scientist to join Discovery Immunology’s Nonhuman Primate (NHP) In Vivo Pharmacology team. As part of that team, this position will drive the development and deployment of NHP in vitro and in vivo models to support AbbVie’s Immunology drug discovery efforts. This is a hands-on and lab-based role requiring significant cell biology and ex vivo expertise. The ideal candidate will have extensive experience with primary cell cultures and in vitro cell-based assays in the context of autoimmune disease.

Requirements

Bachelor’s Degree at least 7 years of experience, or Master’s Degree with at least five years of experience.
Experience working with human tissue samples and with isolating and characterizing cells such as epithelial, fibroblast, endothelial, and/or immunological (B cells, T cells, monocytes) by methods such as tissue digestion and dissociation.
Extensive experience with primary cell cultures and in vitro cell based assays.
Demonstrated proficiency in cell and molecular biological assays such as qPCR analysis, multicolor flow cytometry, FACS, ELISA/Luminex/MSD, cell proliferation/viability assays, cytotoxicity, and in vitro cell stimulation assays
Strong written/oral communication and presentation skills are important.
Experience with statistical analysis and graphing software (Excel, FlowJo, GraphPad Prism)
Ability to build relationships and work collaboratively with cross-functional teams in a highly matrixed organization.

Nice To Haves

Experience with electronic lab notebooks is preferred.

Responsibilities

Routine culture and maintenance of immortalized and primary cell cultures, especially of NHP and human origin.
Isolation of primary human cells from various sources including tissue and whole blood.
Work with a Principal Scientist to develop flow cytometry panels for immunophenotyping
Design, perform, and interpret in vitro experiments to support drug discovery and development activities with both human and NHP primary immune cells.
Conduct experiments with a high level of independence and initiative and performing tasks with minimal direct supervision for routine methods.
Independently schedule and prioritize workload to manage several tasks simultaneously
Maintain detailed and timely electronic documentation to ensure adherence to departmental procedures and contribute to final reports as needed.
Interact with other scientists within a matrixed environment including to identify / utilize resources outside of own group and presenting findings to audiences with diverse backgrounds (e.g., immunologists, cell biologists, pathologists, bioengineers).