Scientist II, Cell Line Development

Resilience • Mississauga, ON

4d

About The Position

A career at Resilience is more than just a job – it’s an opportunity to change the future. Resilience is a technology-focused biomanufacturing company that’s changing the way medicine is made. We’re building a sustainable network of high-tech, end-to-end manufacturing solutions to better withstand disruptive events, serve scientific discovery, and reach those in need. The Scientist II (Molecular and Cellular Biology) is a technical expert who will lead cell line development in support of manufacturing projects for biopharmaceutical products at RBI. The current role requires leading the design, engineering, and optimization of mammalian cell lines for the production and manufacturing of recombinant biopharmaceutical therapeutic proteins (e.g., monoclonal antibodies, bispecific antibodies, fusion proteins). The position involves designing and preparing expression vector constructs (DNA plasmids); incorporation of genetic constructs into host cells (e.g., transfection; CHO cells); selection and creation of stable clonal pools; clone screening and selection; research cell bank (RCB) generation and characterization, cell line stability and characterization. The role also provides technical support, training, and troubleshooting to MSAT and Manufacturing Operations on matters related to master cell bank (MCB) and working cell bank (WCB) testing and characterization. The Scientist II would work closely with third party cell line and/or vector providers as well as with clients in the design and characterization of all aspects of cell line development. The position requires good documentation practices and routine reporting and communication of results, both internally as well as to clients. The role requires the application of advanced expertise and techniques to design and establish well-characterized, industry best practice, cGMP compliant, manufacturing clonal cell lines.

Requirements

• Deep knowledge of recombinant protein expression, molecular and cell biology, cell line development and characterization
• Expert at cell culture techniques with small (shake flask)
• Operational knowledge and hands-on experience with various cell line development instrumentation, e.g., Solentim VIPS, Cell Metric, Octet, CEDEX, BPFlex, Ambr250
• Data analysis and report preparation with knowledge of MS Word, Excel, PowerPoint and Outlook
• Demonstrated ability to succeed in a fast-paced environment, working on multiple projects with constantly changing priorities and deadlines
• Knowledge of aseptic techniques
• Excellent technical problem solver, critical thinker
• Works well independently and in a team environment
• Excellent written and verbal communication skills

Nice To Haves

• Ph.D. or M.Sc. degree in Molecular Biology, Cell Biology, Biochemical Engineering
• Minimum of 5 years (7 years if M.Sc.) of recent scientific and industrial experience in a biotechnology development and manufacturing environment
• Knowledge and experience with routine biologics analytical testing: HPLC, CE-SDS, ELISA, plate-based assays
• Expert at cell culture techniques with bench scale (bioreactors)

Responsibilities

• Conduct cell line development activities for mammalian cell culture manufacturing projects at RBI
• Ensure that all ongoing cell line development activities meet the expected target dates and deliverables
• Train and supervise technical staff in methods and procedures
• Strengthen process and product knowledge
• Resource scheduling, task-tracking and client/vendor interaction
• Maintain strong technical expertise in the full scope of therapeutic protein cell line development covering vector construction (DNA plasmids), transfection (or other means) into host cell line, clone generation, screening and selection (imaging), cell line stability (genetic) and characterization (sequencing), and associated analytics (titer, growth characteristics, metabolites analysis, etc.)
• Prepare Process Designs, Outlines, Workplans, Batch Records, Reports and other required documents for process development work
• Participate in technology transfer activities from clients
• Execute experiments, analyze data, and report results
• Identify, offer, and implement contingency plans to prevent and overcome issues; perform method troubleshooting; identify issues, solve problems and incorporate changes into ongoing development experiments
• Participate in project team and client meetings; present plans and results as appropriate
• Manage outsourced services related to cell line/clone characterization and testing as required
• Present results, recommendations and future plans to management and clients as required
• Lead and participate in project teams and interface with other departments when required (e.g., Manufacturing Operations, Quality Assurance)
• Manage inventory control and tracking of material generated from processes
• Familiar and compliance with Good Documentation Practices (GDP) and Good Laboratory Practices (GLP)
• Identify, review and recommend continuous quality improvements
• Recommend, acquire and maintain equipment suitable for current industry standard cell line development work
• Keep current on industry best practices, standards, and technology pertaining to cell line development instrumentation, analytical techniques, and equipment
• In areas of health, safety and environmental policies, contribute toward meeting departmental/company goals
• Routine maintenance of cell lines, RCBs; support maintenance of MCBs and WCBs; this includes the documentation review of client cell bank analytical and safety testing prior to receipt at RBI’s facility

Benefits

• annual cash bonus program
• RRSP/DPSP with a generous company match
• very competitive healthcare, dentalcare, vision care, life, LTD, and AD&D benefits package
• fertility healthcare and family-forming benefits
• paid vacation
• paid holidays
• pregnancy and parental leave top-up plan
• tuition reimbursement
• employee referral program

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level