Scientist I/Scientist II, tLNP Formulation and Delivery
About The Position
Profluent is seeking a talented, self-motivated, and highly resourceful Scientist I / Scientist II to serve as a foundational scientist in establishing our next-generation Targeted Lipid Nanoparticle (tLNP) platform. This role is central to our strategy of achieving extrahepatic tissue tropism for next-generation genetic medicines. In this position, you will own the end-to-end design, formulation, bioconjugation, and physicochemical characterization of novel tLNPs. You will establish our internal in vitro cell culture and assay pipeline to validate targeting and cellular uptake, while serving as the primary technical interface for in vivo studies. This is a high-visibility, high-impact role ideal for an agile scientist with strong drug delivery foundations who thrives in an entrepreneurial environment.
Requirements
- Scientist I: Ph.D. in Biomedical Engineering, Chemical Engineering, Pharmaceutical Sciences, or a related field with 0–2 years of relevant experience. Scientist II: Ph.D. in the same disciplines with 2–4 years of relevant experience.
- Hands-on experience with LNP assembly platforms (microfluidic/NanoAssemblr, T-junction, or ethanol injection) and surface bioconjugation techniques to functionalize nanoparticles with targeting moieties.
- Demonstrated understanding and experience in shifting nanoparticle biodistribution/tropism through lipid compositional tweaking, molar ratio modification, or structural lipid alteration.
- Hands-on proficiency in mammalian cell culture and running quantitative cell-based assays (Flow Cytometry/FACS, reporter assays, ELISAs).
- Understanding of systemic nanoparticle distribution, protein corona formation, vascular barriers, and in vivo study design principles. (Hands-on animal dosing or ex vivo tissue processing experience is not required).
- Entrepreneurial drive and organizational skills suited for building assays, protocols, and workflows in an early-stage, growing R&D environment.
Nice To Haves
- Experience with automated or high-throughput liquid-handling platforms for formulation screening.
- Practical knowledge of Design of Experiments (DoE) software (e.g., JMP) for systematic library screening.
- Experience with advanced nanoparticle characterization techniques (e.g. HPLC-SEC, UPLC-CAD).
Responsibilities
- Design and execute tLNP formulations encapsulating nucleic acid payloads (e.g., mRNA, sgRNA), systematically altering lipid ratios, ionizable lipid structures, and helper components to drive extrahepatic tissue tropism.
- Perform hands-on surface modifications and bioconjugation chemistries (e.g., maleimide-thiol, click chemistry, enzymatic tags) to attach targeting moieties (antibodies, VHHs/nanobodies, peptides) to LNP surfaces.
- Perform routine and advanced LNP analytics, including particle size/polydispersity (e.g., dynamic light scattering), zeta potential and encapsulation efficiency (e.g., RiboGreen assays) and Degree of Labeling (DoL) for conjugated ligands.
- Establish and maintain mammalian cell culture models to support platform screening needs. Develop, optimize, and execute robust in vitro assays (using Flow Cytometry/FACS, plate readers, and fluorescence/luminescence imaging) to evaluate target binding, receptor-mediated cellular uptake, endosomal escape, and functional cargo translation.
- Design robust in vivo biodistribution, pharmacokinetic (PK), and organ-targeting studies to evaluate tLNP performance. Analyze, interpret datasets to establish clear structure-activity relationships (SAR) for the next round of formulation design.
- Maintain detailed Electronic Lab Notebook (ELN) records, author technical reports, generate patent disclosure data, and present findings to cross-functional scientific leadership.
Benefits
- Competitive compensation package with equity participation
- 401(k) with a strong employer match
- Comprehensive benefits including health/dental/vision insurance
- Generous PTO policy and commitment to work-life balance
- Professional development opportunities in a cutting-edge field at the intersection of AI and biology
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What This Job Offers
Job Type
Full-time
Career Level
Entry Level
Education Level
Ph.D. or professional degree
