Scientist I, Immunology

Generate Biomedicines•Somerville, MA

21h

About The Position

Generate:Biomedicines is seeking a Scientist I with a strong background in immunology, particularly T cell immunobiology, and hands-on expertise working with human primary immune cells to support the development of protein-based therapeutics for immune-mediated diseases. As a member of a multidisciplinary team, this individual will independently plan and execute in vitro experiments, analyze and interpret results, and generate robust data packages that inform project decisions and pipeline progression. This highly laboratory-focused role offers the opportunity to apply mechanistic immunology to the development of AI-enabled biologic therapeutics. While the core activities will focus on immunology, a curious scientist eager to broaden their experience in drug discovery and development will have opportunities to contribute to other therapeutic areas.

Requirements

Ph.D. in immunology, cell biology, or a related field; or a master’s degree in a related field with six years of relevant experience; or a bachelor’s degree in a related field with eight years of relevant experience; or eleven years of relevant experience
Strong understanding of T cell immunobiology, including activation, differentiation, effector functions, and mechanisms of immune regulation
Hands-on expertise in human primary immune-cell isolation, culture, and stimulation, including PBMCs and purified T cells, together with proficiency in mammalian cell-line culture, aseptic technique, and cell quality control
Experience with multicolor, high-parameter flow cytometry, including panel design and optimization, staining, appropriate controls, data acquisition, and analysis
Experience with quantitative immunoassays for cytokines and other proteins, such as ELISA, AlphaLISA, or Meso Scale Discovery (MSD) assays
Proficiency in general molecular biology and biochemistry techniques, such as PCR/qPCR, molecular cloning, and Western blotting
Experience developing, optimizing, or validating cell-based functional assays involving antibodies or other functional proteins
Ability to independently troubleshoot experiments, analyze quantitative data, and draw scientifically sound conclusions within a multidisciplinary project team

Nice To Haves

Industry experience in biologics discovery or development, particularly for inflammatory or autoimmune diseases
Experience with additional human primary immune-cell populations, such as B cells, monocytes, macrophages, or dendritic cells, or with patient-derived samples
Experience with high-throughput screening, laboratory automation, cell line engineering, or in vivo models relevant to immunology or immuno-oncology

Responsibilities

Design, execute, and troubleshoot cell-based assays using human primary peripheral blood mononuclear cells (PBMCs), purified T cells, and other relevant immune-cell populations to characterize the activity and mechanism of action of Generate’s protein therapeutics
Develop and optimize T cell functional assays and co-culture systems to assess activation, proliferation, differentiation, cytokine production, and immune regulation
Apply high-parameter flow cytometry, quantitative immunoassays, and molecular biology and biochemistry techniques to characterize immune-cell phenotypes and functional responses
Plan experiments with appropriate controls and consideration of donor variability, and analyze and interpret reproducible data to support project decisions
Collaborate with cross-functional partners in Automation, Computational Sciences, Protein Sciences, and High-Throughput Screening to advance shared project goals
Maintain complete and accurate experimental records, present findings and recommendations to project teams, and stay current with relevant advances in immunology and biologics research