Scientist, DNA Construct & Plasmid Map QC

Systimmune • Bothell, WA

15d • $90,000 - $115,000 • Onsite

About The Position

SystImmune is seeking a hands-on molecular biologist to lead DNA construct design, high-throughput cloning, and sequence quality control for its AI-driven antibody and protein discovery platform. The role delivers sequence-verified constructs for research and expression applications and maintains accurate, traceable records connecting molecular designs to physical samples. This is a full-time onsite position in Bothell, Washington.

SystImmune is a clinical-stage biopharmaceutical company developing cancer treatments, including bispecific and multispecific antibodies and antibody-drug conjugates. Its pipeline includes clinical-stage assets and preclinical programs. The position partners with protein science and automation teams to transfer verified constructs into expression workflows and improve reproducibility and throughput.

Requirements

• PhD in molecular biology, biochemistry, or a related field.
• 0–3 years of relevant industry experience.
• Strong practical expertise in DNA construct and primer design, molecular cloning, and troubleshooting, including proficiency in at least two assembly or cloning approaches.
• Proficiency in Benchling, Geneious, or comparable software for sequence analysis, alignment, plasmid annotation, and construct record management.
• Experience interpreting sequencing results, identifying sequence errors, and maintaining accurate plasmid maps and sample records.
• Experience with parallel or plate-based cloning workflows.
• Strong attention to detail, clear documentation, and cross-functional communication.

Nice To Haves

• Experience with 96-well cloning, whole-plasmid NGS analysis, or liquid-handling automation.
• Antibody construct design across formats such as Fab, IgG, VHH, scFv, and multispecific proteins.
• Python or other scripting skills for sequence processing, QC, or workflow automation.
• Familiarity with protein purification and characterization, including ÄKTA, SEC, BLI, or SPR.

Responsibilities

• Design expression constructs and primers, selecting appropriate vectors, regulatory elements, tags, and codon usage for the intended expression system.
• Execute and troubleshoot cloning, assembly, and site-directed mutagenesis using methods such as Gibson assembly, Golden Gate, restriction–ligation, and In-Fusion.
• Develop and scale plate-based cloning workflows, including bacterial transformation, colony screening, plasmid preparation, and sequence verification.
• Review Sanger and whole-plasmid sequencing data against intended designs, investigate sequence discrepancies, and document construct release decisions.
• Maintain annotated, version-controlled plasmid maps and sequence records in Geneious with traceability to sample IDs and storage locations.
• Establish naming conventions, annotation standards, QC acceptance criteria, and SOPs; monitor cloning success and turnaround time.
• Partner with protein science and automation teams to transfer verified constructs into expression workflows and improve reproducibility and throughput.

Benefits

• 100% paid employee premiums for medical, dental, and vision insurance.
• Short-term disability insurance.
• Long-term disability insurance.
• 401(k) plan with a 50% company match of up to 3%, with a five-year vesting schedule.
• 15 PTO days per year.
• Sick leave.
• 11 paid holidays.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level
• Education Level: Ph.D. or professional degree