Scientist Antibody Discovery

St. Jude Children's Research Hospital•Memphis, TN

2d•$86,320 - $154,960

About The Position

Join an excellent team of researchers dedicated to coming closer to the mission of St. Jude Children’s Research Hospital, that no child will die at the dawn of life. Antibody Discovery Collaborative Centre (ADCC) within the Department of Host Microbe Interactions at St. Jude seeks a skilled and highly motivated candidate experienced in antibody discovery. Candidates with experience in antibody discovery, protein engineering OR expression and validation of biologics are encouraged to apply.

Requirements

Candidates with experience in antibody discovery, protein engineering OR expression and validation of biologics are encouraged to apply.
Previous experience in integrating ML-based approaches with display methods would be preferred.
Previous experience in library generation would be vital.
Bachelor's degree in relevant scientific area is required.
Eight (8) years of relevant work experience is required
Six (6) years of relevant work experience is required with a Master's degree
Three (3) years of relevant work experience is required with a PhD

Nice To Haves

Experience with merging cryo-EM techniques with epitope analysis of antibody repertoires
Experience with high-throughput automated protein purification systems (e.g. Kingfisher)
Experience with antibody-related bioinformatic/software packages.
Expertise in high-throughput molecular biology approaches for construct and library generation.
Experience with mAb characterization techniques (FACS, BLI, epitope binning).

Responsibilities

Antibody discovery: Focus on display methods (phage, yeast, mammalian) and experience in building high-diversity libraries from synthetic and animal repertoires.
Experienced in designing discovery workflows and delivering panel of lead candidates for programs.
Apply in-vitro display methods for sequence discovery and ability to handle multiple screening arms for antigens.
Apply NGS methods to mine antibody pools for sequence discovery
Develop and optimize high-throughput mammalian cell-based production platforms for recombinant antibodies, antigens, and other biologics
Work closely with mAb discovery platforms to support molecular biology workflows; design and execute construct generation for antibody expression.
Perform DNA preparations to generate high-quality plasmid material for mammalian cell transfection.
Establish scalable transient and stable expression workflows in CHO, HEK293, and other mammalian expression systems.
Purification using AKTA chromatography systems.
High-Performance Liquid Chromatography Size Exclusion Chromatography (HPLC-SEC) analysis of purified proteins.
Molecular assessment of early-stage development candidates (polyreactivity ELISA, CE-SDS, SEC-MALS, DSF)
Maintain accurate and timely ELN data