Research Technologist - Molecular Medicine/PKG Research

Mayo Clinic•Rochester, MN

5d•Onsite

About The Position

This position opens for a project focused on comprehending the pathogenesis of polycystic kidney disease, the #1 monogenic human disease leading to kidney failure and death. We integrate cultured cell lines, organoids, and animal models to understand how primary cilia regulate the formation of kidney cysts during development and aging. We welcome individuals who are highly curious to molecular mechanisms underlying human diseases and highly motivated and committed to explorative mechanistic research. Common molecular and cellular techniques are required, including molecular cloning, mutagenesis, cell culture and transfection, and immunoblotting. Experience on recombinant protein expression and pull-down assays would be beneficial. Candidates familiar with various microscope imaging techniques especially immunofluorescence microscopy are highly encouraged to apply. The project also involves proteomics studies, as well as investigating disease phenotype in established mouse models using MRI imaging and histological analyses. This lab is affiliated with the Mayo Clinic Translational PKD Center and closely collaborates with its world-renowned PKD researchers and physicians. Succeeding candidate will have full access to research tools and the state-of-the-art technical services available at the PKD Center, including patient samples and disease models that can be used for pre-clinical studies if promising drug targets are defined. While we greatly appreciate the independent problem-solving ability that is critical for research, we do recognize the uniqueness of each lab member and encourage teamwork and collaboration, devoting to cultivate a supportive environment to promote personal growth.

Requirements

High curiosity for molecular mechanisms underlying human diseases
High motivation and commitment to explorative mechanistic research
Proficiency in common molecular and cellular techniques including molecular cloning, mutagenesis, cell culture and transfection, and immunoblotting

Nice To Haves

Experience on recombinant protein expression and pull-down assays
Familiarity with various microscope imaging techniques, especially immunofluorescence microscopy
Independent problem-solving ability

Responsibilities

Comprehending the pathogenesis of polycystic kidney disease
Integrating cultured cell lines, organoids, and animal models to understand how primary cilia regulate the formation of kidney cysts during development and aging
Performing molecular cloning, mutagenesis, cell culture and transfection, and immunoblotting
Conducting recombinant protein expression and pull-down assays
Utilizing microscope imaging techniques, especially immunofluorescence microscopy
Performing proteomics studies
Investigating disease phenotype in established mouse models using MRI imaging and histological analyses