Research Technologist - Molecular Medicine/PKG Research

Mayo Clinic•Rochester, MN

5d•Onsite

About The Position

This position opens for a project focused on comprehending the pathogenesis of polycystic kidney disease, the #1 monogenic human disease leading to kidney failure and death. We integrate cultured cell lines, organoids, and animal models to understand how primary cilia regulate the formation of kidney cysts during development and aging. We welcome individuals who are highly curious to molecular mechanisms underlying human diseases and highly motivated and committed to explorative mechanistic research. Common molecular and cellular techniques are required, including molecular cloning, mutagenesis, cell culture and transfection, and immunoblotting. Experience on recombinant protein expression and pull-down assays would be beneficial. Candidates familiar with various microscope imaging techniques especially immunofluorescence microscopy are highly encouraged to apply. The project also involves proteomics studies, as well as investigating disease phenotype in established mouse models using MRI imaging and histological analyses. This lab is affiliated with the Mayo Clinic Translational PKD Center and closely collaborates with its world-renowned PKD researchers and physicians. Succeeding candidate will have full access to research tools and the state-of-the-art technical services available at the PKD Center, including patient samples and disease models that can be used for pre-clinical studies if promising drug targets are defined. While we greatly appreciate the independent problem-solving ability that is critical for research, we do recognize the uniqueness of each lab member and encourage teamwork and collaboration, devoting to cultivate a supportive environment to promote personal growth.

Requirements

Bachelor's degree in biology, chemistry, other relevant science, or an associate degree with 6 years of relevant laboratory experience.
Solid understanding of lab techniques, equipment, and safety.
Ability to organize and carry out laboratory techniques independently and in a team setting.
Experience with basic data analysis.
Demonstrated writing skills.
Common molecular and cellular techniques including molecular cloning, mutagenesis, cell culture and transfection, and immunoblotting.

Nice To Haves

Experience on recombinant protein expression and pull-down assays.
Familiarity with various microscope imaging techniques, especially immunofluorescence microscopy.
Reasonable level of experience in a biochemistry/molecular biology laboratory.
Prior experience with cell culture and animal studies.
Enthusiastic individual who works well as part of a team, is task oriented, and is committed to the vision of our research program.

Responsibilities

Integrate cultured cell lines, organoids, and animal models to understand how primary cilia regulate the formation of kidney cysts during development and aging.
Perform molecular cloning, mutagenesis, cell culture and transfection, and immunoblotting.
Conduct recombinant protein expression and pull-down assays.
Utilize various microscope imaging techniques, especially immunofluorescence microscopy.
Engage in proteomics studies.
Investigate disease phenotype in established mouse models using MRI imaging and histological analyses.
Collaborate with researchers and physicians at the Mayo Clinic Translational PKD Center.
Utilize research tools and technical services available at the PKD Center, including patient samples and disease models.
Solve research problems independently and work collaboratively in a team setting.
Organize and carry out laboratory techniques independently and in a team setting.
Perform basic data analysis.
Demonstrate writing skills.