Research Technologist II Ophthalmology & Visual Sciences

Medical College of Wisconsin • Milwaukee, WI

5d • Onsite

About The Position

The applicant will join the Miesfeld laboratory at the Medical College of Wisconsin Eye Institute and perform research tasks under the guidance of Dr. Joel B. Miesfeld. The research performed in the Miesfeld lab explores the gene regulatory mechanisms controlling retinal development, disease and regeneration, with an emphasis on retinal ganglion cells (RGCs), the pathogenic target in Glaucoma and conduit connecting the eye to the brain. The laboratory combines a variety of techniques (biochemistry, histochemistry, molecular biology, microscopy, CRISPR/Cas9 genome editing, etc.) and model systems (mouse, zebrafish, tissue culture) to elucidate the mechanisms responsible for retinal homeostasis. As a member of the Miesfeld lab the applicant will assist research staff in the everyday running of the lab and provide research support to further our understanding of eye development, disease, and vision loss prevention.

Requirements

• Knowledge of biology, chemistry, mathematics, documentation, and records management.
• Data utilization, complex problem solving, critical thinking, resource management, and writing skills.
• Bachelor’s degree
• 1 year of mouse experience, which includes handling, colony management, tissue collection, and genotyping.
• Mouse experience can be substituted for zebrafish experience.
• Standard molecular biology techniques such as molecular cloning and PCR.
• Making of standard stock solutions and dilutions.

Nice To Haves

• Mouse and zebrafish handling/colony management experience which includes tissue collection for genotyping.
• Zebrafish microinjection and tissue collection.
• Mouse tissue collection.
• Cryosectioning and immunohistochemistry.
• Microscopy (confocal or widefield).
• Image processing.
• Microdissection techniques.

Responsibilities

• Assist in the breeding and PCR genotyping of mutant/transgenic mice and zebrafish.
• Conduct molecular biology work, including plasmid tool assembly via cloning.
• Collect and process tissue for bio- and histochemical analysis and subsequent data collection (microscopy, protein detection, high-throughput DNA/RNA sequencing).
• Carry out routine laboratory duties, including the washing and sterilizing of glassware and equipment, disposal of laboratory waste, ordering and inventory of laboratory supplies, and coordinating shipping of materials to and from research collaborators.
• Maintain complete, accurate records and summaries of all experimental results and data generated.
• Assist with the evaluation, interpretation and assessment of experimental data and quality control assays.
• Participate in meetings with principal investigator and research staff.
• Complete all necessary formal training requirements (e.g. health and safety training) promptly.
• Oversee the training of other lab personnel
• Other duties assigned

Benefits

• Outstanding Healthcare Coverage, including but not limited to Health, Vision, and Dental.
• Flexible Spending options
• 403B Retirement Package
• Competitive Vacation and Paid Holidays offered
• Tuition Reimbursement
• Paid Parental Leave
• Employee & Family Assistance Program (EFAP)
• Pet Insurance
• On campus Fitness Facility, offering onsite classes
• Additional discounted rates on items such as: Select cell phone plans, local fitness facilities, Milwaukee recreation and entertainment etc.

What This Job Offers

• Job Type: Full-time
• Career Level: Mid Level