Research Technician

University of Chicago•Chicago, IL

1d•$50,000 - $70,000•Onsite

About The Position

The Wolf lab focuses on the characterization of the effective and failing T cell receptor (TCR)-repertoire to identify therapeutic TCR candidates for potential clinical translation. The gained insights can be applied to various immunotherapies since our research investigates the TCR-response of the host, which is the foundation to understand and utilize an effective immune response against any target. We study pre-clinical mouse models as well as human patient samples to translate findings from bench to bedside and back again. The main aim is advancing individualized TCR-T cell therapy against any type of cancer. To achieve this, the long-term goal is building a platform that allows the selection and validation of putative therapeutic TCR candidates. However, insights will also improve our understanding of various other immunotherapeutic interventions using vaccinations, tumor infiltrating lymphocytes (TILs) or immune checkpoint blockade (ICB).

Requirements

Minimum requirements include a college or university degree in related field.
Minimum requirements include knowledge and skills developed through < 2 years of work experience in a related job discipline.

Nice To Haves

Bachelor's degree.
Handling and working with mice.
Familiar with cell culture and molecular biology techniques, including DNA isolation, PCR, gel electrophoresis.
Basic knowledge of research techniques and methods.
Basic office/lab software skills, including Microsoft Office, Excel, and Power Point.
Organized, task-oriented, good record keeper.
Excellent organizational skills, problem-solving skills, and attention to detail.
Strong verbal and written communication skills.
Work with little direct oversight and within a larger research team.
Adaptable and willing to learn new skills and take on new responsibilities.

Responsibilities

Works on a research project with guidance from the principal investigator and other laboratory members.
Designs and executes daily laboratory experiments, including mammalian cell culture, 3D organoid culture, molecular biology techniques (PCR, cloning), RNA/DNA isolation, and immunological assays such as ELISA and flow cytometry.
Keeps notes and records experimental data.
Works with mouse models, including breeding of mice, weaning pups, genotyping, and phenotyping.
Assists in management of mouse colony.
Receives, labels and safely stores lab supplies.
Maintains and renews inventory of laboratory supplies and equipment.
Assists in training students and new laboratory personnel in techniques and equipment operation.
Maintains technical and administrative support for a research project.
Analyzes and maintains data and/or specimens. Conducts literature reviews. Assists with preparation of reports, manuscripts and other documents.
Performs other related work as needed.