Research Technician

Vanderbilt University • Nashville, TN

1d • Onsite

About The Position

The Research Technician will be part of the Weaver laboratory in the Cell and Developmental Biology Department at Vanderbilt University. This Research Technician is responsible for extracellular vesicle (EV) purifications and quality control analysis, development of new cell lines for cancer-related EV studies, and sample processing for downstream analyses (imaging, proteomics, lipidomics, RNA sequencing). Much of the work relates to a project on how RNA is trafficked into EVs and how those RNA-containing EVs drive colon cancer aggressiveness.

Requirements

• A Bachelor’s degree in biology, chemistry, or a related field of study from an accredited institution of higher education is required.
• Experience performing adherent mammalian cell culture is required.
• Strong organizational, oral, and written communication skills are required.

Nice To Haves

• Experience with biochemical isolation and purification is preferred.
• Experience performing biochemical purification is preferred.
• At least one consecutive year of related lab experience is preferred, either in undergraduate research or in a post-graduate technical position.

Responsibilities

• Extracellular Vesicle purification and analysis
• Adherent Cell Culture
• Genetic engineering of cell lines
• Carrying out experiments under supervision
• Proactively troubleshooting and seeking out advice/feedback
• Maintaining accurate experimental records
• Meeting at least once a week with Dr. Weaver and/or laboratory subgroups to present data and report on the week’s activities
• Maintaining equipment and reagent stocks
• Organizing laboratory protocols in shared online databases
• CRISPR-based genetic engineering and culturing of adherent mammalian cell lines
• Biochemical purification and characterization of EVs
• Mouse tissue harvesting and processing
• Western blotting and other experimental techniques in support of ongoing research projects
• Work alongside other research staff in the laboratory to facilitate ordering, equipment maintenance and training, and laboratory organization.

Benefits

• Vanderbilt University is an equal-opportunity employer. All qualified applicants will receive consideration for employment without regard to race, color, religion, sex, sexual orientation, gender identity, national origin, disability, or status as a protected veteran, or any other characteristic protected by law.

What This Job Offers

• Job Type: Full-time
• Career Level: Entry Level